Prevalence of CTX-M beta-Lactamases Producing Multidrug Resistant Escherichia coli and Klebsiella pneumoniae among Patients Attending Bir Hospital, Nepal
文献类型：期刊论文
| 作者 | Koirala, Sushma; Khadka, Sujan; Sapkota, Sanjeep; Sharma, Suprina; Khanal, Santosh; Thapa, Alina; Khadka, Dhruba Kumar; Poudel, Pramod |
| 刊名 | BIOMED RESEARCH INTERNATIONAL
 |
| 出版日期 | 2021-06-09 |
| 卷号 | 2021页码:- |
| ISSN号 | 2314-6133 |
| 英文摘要 | The emergence of multidrug resistant (MDR) bacteria which is attributable to extended spectrum beta-lactamases (ESBLs) production of CTX-M types is an obvious problem worldwide. This study is aimed at determining the prevalence of CTX-M beta-lactamases producing multidrug resistant Escherichia coli and Klebsiella pneumoniae among patients attending Bir Hospital. A cross-sectional study was conducted between April and September 2019 at Bir Hospital, Kathmandu, and Department of Microbiology, National College, Kathmandu, Nepal. A total of 5,690 different clinical specimens were subjected to cultural, microscopic, and biochemical analyses for the identification of the isolates. Antimicrobial susceptibility testing of the isolates was done, and MDR isolates were selected and processed for further ESBL confirmation by the combination disks method. All confirmed ESBL isolates were screened for CTX-M type beta-lactamases (bla(CTX-M)) by PCR. Of the total 345 isolates (227 Escherichia coli and 118 Klebsiella pneumoniae), 232 were MDR. All 232 (67.24%) MDR isolates were suspected as ESBL producers on the screening test. However, on the phenotypic test, 135 (58.18%) of total MDR bacteria were confirmed as ESBL producers with the highest proportion in K. pneumoniae (59.37%). The major source of ESBL producers was urine. ESBL producing isolates were mostly identified from outpatients and patients belonging to age group 41-60. Gentamicin was found to be effective against ESBL producers. The prevalence of bla(CTX-M) was (89.62%) with the highest frequency for E. coli (93.81%). High prevalence of ESBL of CTX-M types among MDR E. coli and K. pneumoniae was detected from clinical specimens of patients in Bir Hospital. This study warrants the need for the judicious use of antibiotics as well as emphasize the use of modern diagnostic tools for the early detection of MDR and ESBL producers to curb the emergence and spread of MDR and ESBL producing bacteria in the clinical settings of Nepal. |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45984]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Tribhuvan Univ, Dept Microbiol, Natl Coll NIST, Kathmandu, Nepal 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Peoples R China 5.Chinese Acad Sci, Inst Tibetan Plateau Res, State Key Lab Alpine Ecol & Biodivers, Beijing 100101, Peoples R China 6.Natl Acad Med Sci NAMS, Bir Hosp, Dept Microbiol, Kathmandu, Nepal |
| 推荐引用方式 GB/T 7714 | Koirala, Sushma,Khadka, Sujan,Sapkota, Sanjeep,et al. Prevalence of CTX-M beta-Lactamases Producing Multidrug Resistant Escherichia coli and Klebsiella pneumoniae among Patients Attending Bir Hospital, Nepal[J]. BIOMED RESEARCH INTERNATIONAL,2021,2021:-. |
| APA | Koirala, Sushma.,Khadka, Sujan.,Sapkota, Sanjeep.,Sharma, Suprina.,Khanal, Santosh.,...&Poudel, Pramod.(2021).Prevalence of CTX-M beta-Lactamases Producing Multidrug Resistant Escherichia coli and Klebsiella pneumoniae among Patients Attending Bir Hospital, Nepal.BIOMED RESEARCH INTERNATIONAL,2021,-. |
| MLA | Koirala, Sushma,et al."Prevalence of CTX-M beta-Lactamases Producing Multidrug Resistant Escherichia coli and Klebsiella pneumoniae among Patients Attending Bir Hospital, Nepal".BIOMED RESEARCH INTERNATIONAL 2021(2021):-. |
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来源：生态环境研究中心
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