Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples
文献类型：期刊论文
| 作者 | Thapa, Punam; Thapa, Alina; Khadka, Sujan; Sapkota, Sanjeep; Panta, Om Prakash; Sharma, Suprina; Karki, Tika Bahadur; Poudel, Pramod |
| 刊名 | HELIYON
 |
| 出版日期 | 2021-08 |
| 卷号 | 7期号:8页码:- |
| 关键词 | gamma-PGA Bacillus species Optimization 16S rRNA gene |
| 英文摘要 | Microbially produced gamma poly glutamic acid (gamma-PGA) is a commercially important biopolymer with many applications in foods and various other substances and are abundantly used in different parts of the world. With an aim to study the potent gamma-PGA producing Bacillus species, a total of 47 different samples (Kinema, soil, and water) were randomly collected from different locations across the country, and Bacillus sp. were selectively isolated, screened, and characterized by performing physiological, biochemical, morphological, and 16S rRNA gene sequencing. The microbial production of gamma-PGA was assayed with the selected isolates on the PGA medium and the metabolite obtained was recovered by ethanol precipitation method and further characterized by thin-layer chromatography (TLC). Thermotolerance (25-60 degrees C), pH tolerance (4-9), and NaCl tolerance (1-9%) tests were performed to optimize the bacterial growth and gamma-PGA production and its viscosity were measured by Ostwald's viscometer. Out of 145 randomly selected colonies, 63 isolates were Gram-positive, rods, and endospore producers and were presumptively confirmed as genus Bacillus. Higher growth of gamma-PGA producers were reported in 22 isolates and was found at optimum conditions such as temperature (30-37 C), pH (6.5-7), incubation time(3 days), and NaCl concentration (3%) and gamma-PGA thus produced was further verified by TLC with the retention factor (RF) value 0.27. The potent isolates were closely similar to Bacillus subtilis subsp. stercoris, Bacillus cereus, Bacillus paranthracis, and Bacillus licheniformis etc. Based on the findings of the study, B. licheniformis is the most potent gamma-PGA producing Bacillus sp. which can further be used for the commercial production of gamma-PGA. To the best of our knowledge, there is yet no published research from Nepal showing the production of the gamma-PGA although microbially produced gamma-PGA are the major constituents in some popular foods in particular communities of the country. |
| WOS研究方向 | Multidisciplinary Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45994]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Tribhuvan Univ, Natl Coll NIST, Dept Microbiol, POB 8659, Kathmandu, Nepal 3.Chinese Acad Sci, Inst Tibetan Plateau Res, State Key Lab Alpine Ecol & Biodivers, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Peoples R China 6.Tribhuvan Univ, Cent Dept Microbiol, Kathmandu 44601, Nepal |
| 推荐引用方式 GB/T 7714 | Thapa, Punam,Thapa, Alina,Khadka, Sujan,et al. Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples[J]. HELIYON,2021,7(8):-. |
| APA | Thapa, Punam.,Thapa, Alina.,Khadka, Sujan.,Sapkota, Sanjeep.,Panta, Om Prakash.,...&Poudel, Pramod.(2021).Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples.HELIYON,7(8),-. |
| MLA | Thapa, Punam,et al."Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples".HELIYON 7.8(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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