Class 1 In-Tn5393c array contributed to antibiotic resistance of non-pathogenic Pseudoxanthomonas mexicana isolated from a wastewater bioreactor treating streptomycin
文献类型：期刊论文
| 作者 | Selvaraj, Ganesh-Kumar; Wang, Hanqing; Zhang, Yu ; Tian, Zhe; Chai, Wenbo; Lu, Huijie
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2022-05-15 |
| 卷号 | 821期号:0页码:153537 |
| 关键词 | COMPARATIVE GENOMIC ANALYSIS SP NOV. HORIZONTAL TRANSFER TREATMENT PLANTS GENES REVEALS TRANSPOSONS PREVALENCE TETRACYCLINE DEGRADATION |
| ISSN号 | 0048-9697 |
| 英文摘要 | The emergence of antibiotic resistance in retort to environmental pollutants during wastewater treatment still remains elusive. Here, we first to investigate the emergence of antibiotic resistance in an environmental non-pathogenic bacterium, Pseudoxanthomonas mexicana isolated from a lab-scale bioreactor treating wastewater containing streptomycin. The molecular mechanism of antibiotic resistance development was evaluated in its genomic, transcriptional, and proteomic levels. The streptomycin resistant (SR) strain showed strong resistance to streptomycin (MIC > 600 mu g/mL) as well to sulfamethoxazole, ampicillin, and kanamycin (>= 250 mu g/mL). A 13.4 kb class-1-integron array consisting of a new arrangement of gene cassette (IS6100-sul1-aadA2-catB3-aacA1-2-aadB-int1-IS256-int) linked with Tn5393c transposon was identified in the SR strain, which has only been reported in clinical pathogens so far. iTRAQ-LC-MS/MS proteomics revealed 22 up-regulated proteins in the SR strain growing under 100 mg L-1 streptomycin, involving antibiotic resistance, toxin production, stress response, and ribosomal protein synthesis. At the mRNA level, elevated expressions of ARGs (strA, strB, and aadB) and 30S-ribosomal protein genes (rpsA and rpsU) were observed in the SR strain. The results highlighted the genomic plasticity and multifaceted regulatory mechanism employed by P. mexicana in adaptation to high-level streptomycin during biological wastewater treatment. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47679]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 通讯作者 | Lu, Huijie |
| 作者单位 | 1.Zhejiang Univ, Coll Environm & Resource Sci, Key Lab Environm Remediat & Ecol Hlth, Minist Educ, Hangzhou 310058, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 3.St Peters Inst Higher Educ & Res, Dept Microbiol, Chennai 600054, Tamil Nadu, India |
| 推荐引用方式 GB/T 7714 | Selvaraj, Ganesh-Kumar,Wang, Hanqing,Zhang, Yu,et al. Class 1 In-Tn5393c array contributed to antibiotic resistance of non-pathogenic Pseudoxanthomonas mexicana isolated from a wastewater bioreactor treating streptomycin[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,821(0):153537. |
| APA | Selvaraj, Ganesh-Kumar,Wang, Hanqing,Zhang, Yu,Tian, Zhe,Chai, Wenbo,&Lu, Huijie.(2022).Class 1 In-Tn5393c array contributed to antibiotic resistance of non-pathogenic Pseudoxanthomonas mexicana isolated from a wastewater bioreactor treating streptomycin.SCIENCE OF THE TOTAL ENVIRONMENT,821(0),153537. |
| MLA | Selvaraj, Ganesh-Kumar,et al."Class 1 In-Tn5393c array contributed to antibiotic resistance of non-pathogenic Pseudoxanthomonas mexicana isolated from a wastewater bioreactor treating streptomycin".SCIENCE OF THE TOTAL ENVIRONMENT 821.0(2022):153537. |
入库方式： OAI收割
来源：生态环境研究中心
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