Triclosan is the Predominant Antibacterial Compound in Ontario Sewage Sludge
文献类型：期刊论文
| 作者 | Barrett, Holly; Sun, Jianxian; Gong, Yufeng; Yang, Paul; Hao, Chunyan; Verreault, Jonathan; Zhang, Yu ; Peng, Hui
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2022-05 |
| 期号 | 0页码:1-14 |
| 关键词 | ANTIBIOTIC-RESISTANCE DISRUPTING COMPOUNDS MASS-SPECTROMETRY WASTE-WATER IDENTIFICATION EXTRACTION TARGET GENES CONTAMINANTS TRICLOCARBAN |
| ISSN号 | 0013-936X |
| 英文摘要 | Sewage treatment plants (STPs) accumulate both antibiotic and nonantibiotic antimicrobial compounds that can select for antibiotic resistant bacteria. Herein, we aimed to identify the predominant antibacterial compounds impacting E. coli from Ontario sewage sludge consisting of thousands of unknown compounds. Among the 10 extracted sludge samples, 6 extracts exerted significant growth inhibition effects in E. coli. A total of 103 compounds were tentatively detected across the 10 sludge samples by suspect screening, among which the bacterial enoyl-ACP reductase (FabI) inhibitor triclocarban was detected at the highest abundance. A hypomorphic FabI knockdown E. coli strain was highly susceptible to the sludge extracts, confirming FabI inhibitors as the primary antibacterial compounds in the sludge. Protein affinity pulldown identified tridosan as the major ligand binding to a His-tagged FabI protein from the sludge, despite the higher abundance of triclocarban in the same samples. Effect-directed analysis was used to determine the contributions of tridosan to the observed antibacterial potencies. Antibacterial effects were only detected in F-17 and F-18 across 20 fractions, which was consistent with the elution of tridosan and tridocarban in the same two fractions. Further, potency mass balance analysis confirmed that tridosan explained the majority (58-113%) of inhibition effects from sludge extracts. This study highlighted tridosan as the predominant antibacterial compound in sewage sludge impacting E. coil despite the co-occurrence of numerous other antibiotics and nonantibiotics. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47743]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 通讯作者 | Peng, Hui |
| 作者单位 | 1.Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada 2.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Environm Aquat Chem, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 3.Univ Quebec Montreal, Ctr Rech Toxicol & Environm TOXEN, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada 4.Ontario Minist Environm Conservat & Pk MECP, Toronto, ON M7A 1N3, Canada |
| 推荐引用方式 GB/T 7714 | Barrett, Holly,Sun, Jianxian,Gong, Yufeng,et al. Triclosan is the Predominant Antibacterial Compound in Ontario Sewage Sludge[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022(0):1-14. |
| APA | Barrett, Holly.,Sun, Jianxian.,Gong, Yufeng.,Yang, Paul.,Hao, Chunyan.,...&Peng, Hui.(2022).Triclosan is the Predominant Antibacterial Compound in Ontario Sewage Sludge.ENVIRONMENTAL SCIENCE & TECHNOLOGY(0),1-14. |
| MLA | Barrett, Holly,et al."Triclosan is the Predominant Antibacterial Compound in Ontario Sewage Sludge".ENVIRONMENTAL SCIENCE & TECHNOLOGY .0(2022):1-14. |
入库方式： OAI收割
来源：生态环境研究中心
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