Long-term nitrogen fertilization alters microbial community structure and denitrifier abundance in the deep vadose zone
文献类型：期刊论文
| 作者 | Wang, Fenghua; Chen, Shuaimin; Qin, Shuping; Sun, Ruibo; Zhang, Yuming; Wang, Shiqin; Hu, Chunsheng; Hu, Hangwei; Liu, Binbin |
| 刊名 | JOURNAL OF SOILS AND SEDIMENTS
 |
| 出版日期 | 2021-06 |
| 卷号 | 21期号:6页码:2394-2403 |
| 关键词 | Nitrogen fertilization Vadose zone Microbial community structure Denitrifier Amplicon sequencing |
| ISSN号 | 1439-0108 |
| 英文摘要 | Purpose The excessive use of nitrogen (N) fertilizer in intensive agriculture has increased nitrate leaching into groundwater, but its impacts on N transformation processes and the associated microbial communities in the deep vadose zone remain unclear. Materials and methods Soil samples from 0-1050 cm depth were collected from a 20-year field experiment with two N fertilization treatments: 0 (N0) and 600 kg N ha(-1) year(-1) (N600). Amplicon sequencing and quantitative PCR analyses were performed to profile the vertical distribution of soil microbial communities and denitrification genes. Results and discussion The soil microbial community structure and diversity were strongly influenced by soil depth and N fertilization. The 250 cm depth was identified as a threshold depth, as dramatically different microbial communities were found below and above this depth. Quantitative PCR results showed that the absolute abundance of denitrification genes decreased with increasing soil depth. Conclusion This study elucidated the profound effects of long-term N input on the composition and diversity of the microbial communities and the abundance of denitrifiers in the deep vadose zone. Our results provide basic information for use in mitigating nitrate leaching by enhancing microbial denitrification in deep vadose zones in intensive agricultural areas. |
| WOS研究方向 | Environmental Sciences ; Soil Science |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45505]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 2.Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic 3010, Australia 3.Fujian Agr & Forestry Univ, Coll Resources & Environm, Fujian Prov Key Lab Soil Environm Hlth & Regulat, Fuzhou 350002 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Key Lab Agr Water Resources,Hebei Key Lab Soil Ec, Shijiazhuang 050021, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fenghua,Chen, Shuaimin,Qin, Shuping,et al. Long-term nitrogen fertilization alters microbial community structure and denitrifier abundance in the deep vadose zone[J]. JOURNAL OF SOILS AND SEDIMENTS,2021,21(6):2394-2403. |
| APA | Wang, Fenghua.,Chen, Shuaimin.,Qin, Shuping.,Sun, Ruibo.,Zhang, Yuming.,...&Liu, Binbin.(2021).Long-term nitrogen fertilization alters microbial community structure and denitrifier abundance in the deep vadose zone.JOURNAL OF SOILS AND SEDIMENTS,21(6),2394-2403. |
| MLA | Wang, Fenghua,et al."Long-term nitrogen fertilization alters microbial community structure and denitrifier abundance in the deep vadose zone".JOURNAL OF SOILS AND SEDIMENTS 21.6(2021):2394-2403. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。