Novel clades of soil biphenyl degraders revealed by integrating isotope probing, multi-omics, and single-cell analyses
文献类型：期刊论文
| 作者 | Chen, Song-Can; Budhraja, Rohit; Adrian, Lorenz; Calabrese, Federica; Stryhanyuk, Hryhoriy; Musat, Niculina; Richnow, Hans-Hermann; Duan, Gui-Lan ; Zhu, Yong-Guan; Musat, Florin
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| 刊名 | ISME JOURNAL
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| 页码 | - |
| ISSN号 | 1751-7362 |
| 英文摘要 | Most microorganisms in the biosphere remain uncultured and poorly characterized. Although the surge in genome sequences has enabled insights into the genetic and metabolic properties of uncultured microorganisms, their physiology and ecological roles cannot be determined without direct probing of their activities in natural habitats. Here we employed an experimental framework coupling genome reconstruction and activity assays to characterize the largely uncultured microorganisms responsible for aerobic biodegradation of biphenyl as a proxy for a large class of environmental pollutants, polychlorinated biphenyls. We used C-13-labeled biphenyl in contaminated soils and traced the flow of pollutant-derived carbon into active cells using single-cell analyses and protein-stable isotope probing. The detection of C-13-enriched proteins linked biphenyl biodegradation to the uncultured Alphaproteobacteria clade UBA11222, which we found to host a distinctive biphenyl dioxygenase gene widely retrieved from contaminated environments. The same approach indicated the capacity of Azoarcus species to oxidize biphenyl and suggested similar metabolic abilities for species of Rugosibacter. Biphenyl oxidation would thus represent formerly unrecognized ecological functions of both genera. The quantitative role of these microorganisms in pollutant degradation was resolved using single-cell-based uptake measurements. Our strategy advances our understanding of microbially mediated biodegradation processes and has general application potential for elucidating the ecological roles of uncultured microorganisms in their natural habitats. |
| WOS研究方向 | Ecology ; Microbiology |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45520]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.Tech Univ Berlin, Chair Geobiotechnol, D-13355 Berlin, Germany 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 4.UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, D-04318 Leipzig, Germany 5.UFZ Helmholtz Ctr Environm Res, Dept Environm Biotechnol, Leipzig, Germany |
| 推荐引用方式 GB/T 7714 | Chen, Song-Can,Budhraja, Rohit,Adrian, Lorenz,et al. Novel clades of soil biphenyl degraders revealed by integrating isotope probing, multi-omics, and single-cell analyses[J]. ISME JOURNAL:-. |
| APA | Chen, Song-Can.,Budhraja, Rohit.,Adrian, Lorenz.,Calabrese, Federica.,Stryhanyuk, Hryhoriy.,...&Musat, Florin. |
| MLA | Chen, Song-Can,et al."Novel clades of soil biphenyl degraders revealed by integrating isotope probing, multi-omics, and single-cell analyses".ISME JOURNAL |
入库方式： OAI收割
来源：生态环境研究中心
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