Examining ecological justice within the social-ecological-technological system of New York City, USA
文献类型：期刊论文
| 作者 | Pineda-Pinto, Melissa; Herreros-Cantis, Pablo; McPhearson, Timon; Frantzeskaki, Niki; Wang, Jing ; Zhou, Weiqi
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| 刊名 | LANDSCAPE AND URBAN PLANNING
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| 出版日期 | 2021 |
| 卷号 | 6期号:10页码:3724-3732 |
| 关键词 | phosphorylated histone DNA double-strand breaks electrochemiluminescence immunoassay microplate sensor array |
| ISSN号 | 2379-3694 |
| 英文摘要 | Sensitive detection of phosphorylated histone H2AX (gamma H2AX) in cells as a biomarker of DNA double-strand breaks has great significance in the field of molecular toxicology and life science research. However, current gamma H2AX detection methods require labor- and time-consuming steps. Here, for the first time, we designed a simple electrochemiluminescence (ECL) immunoassay integrated with a microplate-based sensor array to realize sensitive and high-throughput detection of gamma H2AX in cell lysates. Under the optimized conditions, this ECL immunosensor array could linearly respond to gamma H2AX concentrations in the range from 2 x 10(2) to 1 x 10(5) pg/mL. In addition, our approach possessed excellent specificity and satisfactory reproducibility, and its practicality was verified in real cell lysates. The whole process including instrumental and manual operation was completed in no more than 3 h. This study provides a convenient and rapid alternative method for the sensitive quantification of gamma H2AX, which shows promising application in high-throughput screening of genotoxic chemicals and drug candidates. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/46731]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.[Wang, Jing 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Beijing Urban Ecosyst Res Stn, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 5.Zhou, Weiqi 6.Stockholm Univ, Stockholm Resilience Ctr, Stockholm, Sweden 7.Cary Inst Ecosyst Studies, Millbrook, NY USA 8.New Sch Univ, Urban Syst Lab, New York, NY USA 9.New Sch Univ, Urban Syst Lab, 79 5th Ave,16th Floor, New York, NY 10003 USA 10.Swinburne Univ Technol, Fac Hlth Arts & Design, Ctr Urban Transit, Melbourne, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Pineda-Pinto, Melissa,Herreros-Cantis, Pablo,McPhearson, Timon,et al. Examining ecological justice within the social-ecological-technological system of New York City, USA[J]. LANDSCAPE AND URBAN PLANNING,2021,6(10):3724-3732. |
| APA | Pineda-Pinto, Melissa,Herreros-Cantis, Pablo,McPhearson, Timon,Frantzeskaki, Niki,Wang, Jing,&Zhou, Weiqi.(2021).Examining ecological justice within the social-ecological-technological system of New York City, USA.LANDSCAPE AND URBAN PLANNING,6(10),3724-3732. |
| MLA | Pineda-Pinto, Melissa,et al."Examining ecological justice within the social-ecological-technological system of New York City, USA".LANDSCAPE AND URBAN PLANNING 6.10(2021):3724-3732. |
入库方式： OAI收割
来源：生态环境研究中心
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