Impacts of Human Activities on Ecosystem Service Value in Arid and Semi-Arid Ecological Regions of China
文献类型：期刊论文
| 作者 | Fan, Xin; Yu, Haoran; Tiando, Damien Sinonmatohou; Rong, Yuejing; Luo, Wenxu; Eme, Chan; Ou, Shengya; Li, Jiangfeng; Liang, Zhe |
| 刊名 | INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
 |
| 出版日期 | 2021 |
| 页码 | - |
| ISSN号 | 0003-2654 |
| 英文摘要 | Imaging biomolecules within a single bacterial cell is crucial for understanding cellular genetic mechanisms. Herein, we exploited a surface-enhanced Raman spectroscopy (SERS) imaging strategy for single cell analysis. Cellular biosynthesized Ag nanoparticles (NPs) provided the necessary enhancement for SERS imaging. Multiple complementary techniques, including high-resolution transmission electron microscopy (HR-TEM), high-angle annular dark-field (HAADF)-scanning transmission electron microscopy (STEM), and energy-dispersive X-ray spectroscopy (EDX), were used to characterize the biogenic Ag NPs in cells. Three-dimensional SERS imaging maps displayed spectral information of biomolecules within the single cell. The multivariate curve resolution (MCR) model and principal component analysis (PCA) model were used to analyze the cellular SERS imaging maps. The MCR model, with a specific constraint of non-negativity, resulted in meaningful identification of biomolecules associated with Ag reduction. Focusing on the molecular level reveals that Pantoea sp. IMH utilizes several mechanisms to synthesize Ag NPs, including cytoplasm reduction by glucose or nicotinamide adenine dinucleotide (NADH)-dependent reductase, and extracellular reduction by an electron transfer chain containing quinone and cytochrome C. Our results shed new light on the Ag NP biosynthesis mechanism and single cell Raman analysis. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/46738]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.Shaanxi Normal Univ, Sch Geog & Tourism, Xian 710119, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.[Luo, Wenxu 4.China Univ Geosci, Int Educ Coll, Wuhan 430074, Peoples R China 5.China Univ Geosci Wuhan, Sch Publ Adm, Wuhan 430074, Peoples R China 6.Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China 7.Anhui Urbanizat Dev Res Ctr, Hefei 230022, Peoples R China 8.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Xin,Yu, Haoran,Tiando, Damien Sinonmatohou,et al. Impacts of Human Activities on Ecosystem Service Value in Arid and Semi-Arid Ecological Regions of China[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,2021:-. |
| APA | Fan, Xin.,Yu, Haoran.,Tiando, Damien Sinonmatohou.,Rong, Yuejing.,Luo, Wenxu.,...&Liang, Zhe.(2021).Impacts of Human Activities on Ecosystem Service Value in Arid and Semi-Arid Ecological Regions of China.INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,-. |
| MLA | Fan, Xin,et al."Impacts of Human Activities on Ecosystem Service Value in Arid and Semi-Arid Ecological Regions of China".INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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