Quantifying Leaf Trait Covariations and Their Relationships with Plant Adaptation Strategies along an Aridity Gradient
文献类型：期刊论文
| 作者 | Yang, Yanzheng ; Kang, Le; Zhao, Jun; Qi, Ning; Li, Ruonan; Wen, Zhongming; Kassout, Jalal; Peng, Changhui; Lin, Guanghui; Zheng, Hua
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| 刊名 | BIOLOGY-BASEL
 |
| 出版日期 | 2021-10 |
| 卷号 | 287页码:- |
| ISSN号 | 0269-7491 |
| 关键词 | TCDD Macrophage Immunotoxicity Transcriptome WGCNA |
| 英文摘要 | 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a well-known immunotoxic environmental pollutant. However, most immunotoxicology studies of TCDD were based on the animal models and the inner mechanisms have just focused on a few genes/proteins. In this study, the immune functions of THP-1-derived macrophages was measured with in-vitro bioassays after 24-h exposure of TCDD including environmentally relevant concentrations. RNA-seq and Weighted Gene Co-expression Network Analysis were used to characterize the immunotoxicity molecular mechanisms. Our study is the first report on the TCDD-induced effects of cell adhesion, morphology, and multiple cytokines/chemokines production on THP-1 macrophages. After TCDD treatment, we observed an inhibited cell adherence, probably attributed to the suppressed mRNA levels of adhesion molecules ICAM-1, VCAM-1 and CD11b, and a decrease in cell pseudopodia and expression of F-actin. The inflammatory cytokines TNF-alpha, IL-10 and other 8 cytokines/chemokines regulating granulocytes/T cells and angiogenesis were disrupted by TCDD. Alternative splicing event was found to be a sensitive target for TCDD. Using WGCNA, we identified 10 hub genes (TNF, SRC, FGF2, PTGS2, CDH2, GNG11, BDNF, WNT5A, CXCR5 and RUNX2) highly relevant to these observed phenotypes, suggesting AhR less important in the effects TCDD have on THP-1 macrophages than in other cells. Our findings broaden the understanding of TCDD immunotoxicity on macrophages and provide new potential targets for clarifying the molecular mechanisms. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/46748]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 2.Tsinghua Univ, Dept Earth Syst Sci, Minist Educ, Key Lab Earth Syst Modeling, Beijing 100084, Peoples R China 3.[Qi, Ning 4.State Adm Forestry & Grassland, East China Inventory & Planning Inst, Hangzhou 310019, Peoples R China 5.China Aero Geophys Survey, Beijing 100083, Peoples R China 6.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China 7.Remote Sensing Ctr Nat Resources, Beijing 100083, Peoples R China 8.Beijing Forestry Univ, Sch Informat Sci & Technol, Beijing 100083, Peoples R China 9.Abdelmalek Essaadi Univ, Fac Sci, Lab Appl Bot, BioAgrodivers Team, Tetouan 93002, Morocco |
| 推荐引用方式 GB/T 7714 | Yang, Yanzheng,Kang, Le,Zhao, Jun,et al. Quantifying Leaf Trait Covariations and Their Relationships with Plant Adaptation Strategies along an Aridity Gradient[J]. BIOLOGY-BASEL,2021,287:-. |
| APA | Yang, Yanzheng.,Kang, Le.,Zhao, Jun.,Qi, Ning.,Li, Ruonan.,...&Zheng, Hua.(2021).Quantifying Leaf Trait Covariations and Their Relationships with Plant Adaptation Strategies along an Aridity Gradient.BIOLOGY-BASEL,287,-. |
| MLA | Yang, Yanzheng,et al."Quantifying Leaf Trait Covariations and Their Relationships with Plant Adaptation Strategies along an Aridity Gradient".BIOLOGY-BASEL 287(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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