Diverse antibiotic resistance genes and potential pathogens inhabit in the phyllosphere of fresh vegetables
文献类型：期刊论文
| 作者 | Yin, Yue ; Zhu, Dong; Yang, Guang; Su, Jianqiang; Duan, Guilan
|
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2022-04-01 |
| 卷号 | 815期号:0页码:152851 |
| ISSN号 | 0048-9697 |
| 关键词 | ESCHERICHIA-COLI IRRIGATION WATER CROSS-CONTAMINATION SALMONELLA BACTERIA PRODUCE RISK COMMUNITIES RESISTOME SURVIVAL |
| 英文摘要 | Fresh vegetables are considered as a reservoir of pathogenic bacteria and antibiotic resistance genes (ARGs), which are the emerging environmental contaminants, posing increasing concerned risk to human health. However, the prevalence of pathogens in phyllosphere of fresh vegetables, as well as the association of ARGs with pathogenic bacteria, have not been well elaborated. In this study, we explored the structure of microbial communities and ARGs through high-throughput quantitative PCR and 16S rRNA gene Illumina sequencing, and characterized the microorganisms resisting to antibiotics by pure culture. From phyllosphere of six different kinds of vegetables, 205 ARGs were detected and genes for multidrug resistance was the most abundant. The predominant potential pathogens were classified to Pseudomonas, Klebsiella, and Acinetobacter genera, which carried various ARGs such as multidrug and beta-lactam resistance genes presumedly. Among six kinds of vegetables, Lactuca sativa var. asparagina carried the highest abundance of potential pathogens and ARGs, while Allium sativum L harbored the lowest abundance of pathogens and ARGs. In addition, various culturable bacteria resisting to colistin or meropenem could be isolated from all vegetables, remarkably, all the isolates resistant to both antibiotics are potential pathogens. Our study highlighted the risks of pathogens and ARGs from raw vegetables to consumers, characterized their structure patterns among different vegetables, and analyzed the potential mechanisms regulating phyllosphere pathogens and resistome of fresh vegetables, which would be helpful for reducing the microbial risk from vegetable ingestion. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47229]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 通讯作者 | Duan, Guilan |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, State Key Lab Urban & Reg Ecol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Yue,Zhu, Dong,Yang, Guang,et al. Diverse antibiotic resistance genes and potential pathogens inhabit in the phyllosphere of fresh vegetables[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,815(0):152851. |
| APA | Yin, Yue,Zhu, Dong,Yang, Guang,Su, Jianqiang,&Duan, Guilan.(2022).Diverse antibiotic resistance genes and potential pathogens inhabit in the phyllosphere of fresh vegetables.SCIENCE OF THE TOTAL ENVIRONMENT,815(0),152851. |
| MLA | Yin, Yue,et al."Diverse antibiotic resistance genes and potential pathogens inhabit in the phyllosphere of fresh vegetables".SCIENCE OF THE TOTAL ENVIRONMENT 815.0(2022):152851. |
入库方式： OAI收割
来源：生态环境研究中心
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