Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics
文献类型：期刊论文
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| 作者 | Zhu, Lin; Shuai, Xin-Yi; Lin, Ze-Jun; Sun, Yu-Jie; Zhou, Zhen-Chao; Meng, Ling-Xuan; Zhu, Yong-Guan ; Chen, Hong
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| 刊名 | WATER RESEARCH
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| 出版日期 | 2022-02-01 |
| 卷号 | 209期号:0页码:117907 |
| ISSN号 | 0043-1354 |
| 关键词 | RESISTANT BACTERIA COLISTIN RESISTANCE PLASMIDS DIVERSITY INSIGHTS |
| 英文摘要 | Hospital wastewater contains abundant antibiotics, antibiotic resistance genes (ARGs), and pathogens. Lastresort antibiotic resistance genes (LARGs) include the New Delhi metallo-beta-lactamase gene blaNDM, mobile colistin resistance gene mcr and tigecycline resistance gene tet(X) which confers resistance to carbapenems, colistin and tigecycline. The presence and significance of LARGs in hospital wastewater treatment systems (HWTS) have not yet been systematically explored. Here, LARG variants were shown to be prevalent both influents and effluents of HWTS. A total of 989 Enterobacteriaceae isolates that confer resistance to last-resort antibiotics were collected from effluents and multiple genetic contexts of LARGs were analyzed. LARGscarrying plasmids were confirmed to show high multidrug phenotypes and transferability. We also discovered the co-occurrence of plasmids harboring blaNDM-1 and mcr-1 in single Escherichia coli, as well as E. coli HM016 containing two unique mcr-1-carrying plasmids. This result might accelerate co-dissemination of LARGs under environmental selection pressure. Different core genetic arrangements in these strains suggest several evolutionary pathways in HWTS. The resistance functions of LARGs were confirmed in vitro and in vivo by mass spectrometry. This study provides novel insights into the diversity, genetic context and function of critical ARGs in HWTS. The results raise the concern that LARGs may further spread into the environment, thus, more stringent discharge standards and regulations for hospital wastewater are urgently needed. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47292]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 通讯作者 | Chen, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 2.Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou 310058, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Lin,Shuai, Xin-Yi,Lin, Ze-Jun,et al. Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics, Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics.pdf[J]. WATER RESEARCH,2022,209(0):117907. |
| APA | Zhu, Lin.,Shuai, Xin-Yi.,Lin, Ze-Jun.,Sun, Yu-Jie.,Zhou, Zhen-Chao.,...&Chen, Hong.(2022).Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics.WATER RESEARCH,209(0),117907. |
| MLA | Zhu, Lin,et al."Landscape of genes in hospital wastewater breaking through the defense line of last-resort antibiotics".WATER RESEARCH 209.0(2022):117907. |
入库方式： OAI收割
来源：生态环境研究中心
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