Nanopore sequencing analysis of integron gene cassettes in sewages and soils
文献类型：期刊论文
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| 作者 | An, Xin-Li; Abass, Olusegun K.; Zhao, Cai-Xia; Xu, Mei-Rong; Pan, Ting; Pu, Qiang; Liao, Hu; Li, Hu; Zhu, Yong-Guan ; Su, Jian-Qiang
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2022-04-15 |
| 卷号 | 817期号:0页码:152766 |
| ISSN号 | 0048-9697 |
| 关键词 | ANTIBIOTIC-RESISTANCE GENES BACTERIAL COMMUNITIES RECOMBINATION INCREASES DIVERSE SPREAD AGENTS |
| 英文摘要 | Integrons are genetic elements that can facilitate rapid spread of antibiotic resistance by insertion and removal of genes. However, knowledge about the diversity and distribution of gene cassettes embedded in class 1 integron is still limited. In this study, we sequenced integron gene cassettes using nanopore sequencing and quantified antibiotic resistance genes (ARGs) and integrase genes in the manured soils and sewages of a bioreactor. The results showed that class 1 integron integrase genes were the most abundant in soils and sewages compared with class 2 and class 3 integrase genes. Long-term manure application exacerbated the enrichment of total ARGs, integrase genes and antibiotic resistance-associated gene cassettes, while antibiotics and heavymetals showed no impact on the overall resistome profile. Sewage treatment could efficiently remove the absolute abundance of integrase genes (similar to 3 orders of magnitude, copies/L) and antibiotic resistance gene cassettes. The resistance gene cassettes mainly carried the ARGs conferring resistance to aminoglycoside and beta-lactams in soils and sewages, some of which were persistent during the sewage treatment. This study underlined that soil and sewage were potential reservoirs for integron-mediated ARGs transfer, indicating that anthropogenic activity played a vital role in the prevalence and diversity of resistance gene cassettes in integrons. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47304]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Fujian Key Lab Watershed Ecol, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 4.Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore 639798, Singapore 5.Chinese Acad Sci, Inst Geochem, Guiyang 550081, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Xin-Li,Abass, Olusegun K.,Zhao, Cai-Xia,et al. Nanopore sequencing analysis of integron gene cassettes in sewages and soils, Nanopore sequencing analysis of integron gene cassettes in sewages and soils.pdf[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,817(0):152766. |
| APA | An, Xin-Li.,Abass, Olusegun K..,Zhao, Cai-Xia.,Xu, Mei-Rong.,Pan, Ting.,...&Su, Jian-Qiang.(2022).Nanopore sequencing analysis of integron gene cassettes in sewages and soils.SCIENCE OF THE TOTAL ENVIRONMENT,817(0),152766. |
| MLA | An, Xin-Li,et al."Nanopore sequencing analysis of integron gene cassettes in sewages and soils".SCIENCE OF THE TOTAL ENVIRONMENT 817.0(2022):152766. |
入库方式： OAI收割
来源：生态环境研究中心
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