High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants
文献类型：期刊论文
| 作者 | Wei, Ziyan; Feng, Kai ; Wang, Zhujun; Zhang, Yu; Yang, Min; Zhu, Yong-Guan; Virta, Marko P. J.; Deng, Ye
|
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2021-09-07 |
| 卷号 | 55期号:17页码:11824-11834 |
| 关键词 | antibiotic resistance genes horizontal gene transfer vertical gene transfer bacterial hosts epicPCR |
| ISSN号 | 0013-936X |
| 英文摘要 | The spread of antibiotic resistance genes (ARGs) has gained much attention worldwide, while the contribution of vertical gene transfer (VGT) and horizontal gene transfer (HGT) is still elusive. Here, we improved an emerging high-throughput single-cell-based technology, emulsion, paired isolation, and concatenation polymerase chain reaction (epicPCR), by lengthening the sequence of ARG in the fused ARG-16S rRNA fragments to cover the variance of both ARG and its hosts. The improved epicPCR was applied to track the hosts of a widely detected ARG, sul1 gene, in five urban wastewater treatment plants (UWTPs) during two seasons. The sul1 host bacteria were highly diverse and mostly classified as Proteobacteria and Bacteroidetes. Clear seasonal divergence of alpha-diversity and interaction networks were present in the host community. The consensus phylogenetic trees of the sul1 gene and their host demonstrated incorrespondence on the whole and regularity on abundant groups, suggesting the important role of both HGT and VGT, respectively. The relative importance of these two ways was further measured; HGT (54%) generally played an equal or even more important role as VGT (46%) in UWTPs. The application of the improved epicPCR technology provides a feasible approach to quantify the relative contributions of VGT and HGT in environmental dissemination of ARGs. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46118]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Shandong Univ, Inst Marine Sci & Technol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 10008 5.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 6.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, CAS Key Lab Environm Biotechnol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Ziyan,Feng, Kai,Wang, Zhujun,et al. High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(17):11824-11834. |
| APA | Wei, Ziyan.,Feng, Kai.,Wang, Zhujun.,Zhang, Yu.,Yang, Min.,...&Deng, Ye.(2021).High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(17),11824-11834. |
| MLA | Wei, Ziyan,et al."High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.17(2021):11824-11834. |
入库方式： OAI收割
来源：生态环境研究中心
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