Microbial Interactions Drive the Complete Catabolism of the Antibiotic Sulfamethoxazole in Activated Sludge Microbiomes
文献类型：期刊论文
| 作者 | Qi, Mengyuan; Liang, Bin; Zhang, Long; Ma, Xiaodan; Yan, Lei; Dong, Wenchen; Kong, Deyong; Zhang, Liying; Zhu, Haizhen; Gao, Shu-Hong |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2021-03-02 |
| 卷号 | 55期号:5页码:3270-3282 |
| ISSN号 | 0013-936X |
| 英文摘要 | Microbial communities are believed to outperform monocultures in the complete catabolism of organic pollutants via reduced metabolic burden and increased robustness to environmental challenges; however, the interaction mechanism in functional microbiomes remains poorly understood. Here, three functionally differentiated activated sludge microbiomes (S1: complete catabolism of sulfamethoxazole (SMX); S2: complete catabolism of the phenyl part of SMX ([phenyl]-SMX) with stable accumulation of its heterocyclic product 3-amino-S-methylisoxazole (3ASMI); A: complete catabolism of 3ASMI rather than [phenyl]SMX) were enriched. Combining time-series cultivation-independent microbial community analysis, DNA-stable isotope probing, molecular ecological network analysis, and cultivation-dependent function verification, we identified key players involved in the SMX degradation process. Paenarthrobacter and Nocardioides were primary degraders for the initial cleavage of the sulfonamide functional group (-C-S-N- bond) and 3ASMI degradation, respectively. Complete catabolism of SMX was achieved by their cross-feeding. The co-culture of Nocardioides, Acidovorax, and Sphingobium demonstrated that the nondegraders Acidovorax and Sphingobium were involved in the enhancement of 3ASMI degradation. Moreover, we unraveled the internal labor division patterns and connections among the active members centered on the two primary degraders. Overall, the proposed methodology is promisingly applicable and would help generate mechanistic, predictive, and operational understanding of the collaborative biodegradation of various contaminants. This study provides useful information for synthetic activated sludge microbiomes with optimized environmental functions. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46130]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 作者单位 | 1.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Minist Agr,Key Lab Microbiol Agr Environm, Nanjing 210095, Peoples R 2.Univ Canterbury, Dept Civil & Nat Resources Engn, Christchurch 8140, New Zealand 3.Harbin Inst Technol, Sch Environm, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China 4.Harbin Inst Technol Shenzhen, Sch Civil & Environm Engn, Shenzhen 518055, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Biotechnol, Beijing 100085, Peoples R China 6.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Mengyuan,Liang, Bin,Zhang, Long,et al. Microbial Interactions Drive the Complete Catabolism of the Antibiotic Sulfamethoxazole in Activated Sludge Microbiomes[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(5):3270-3282. |
| APA | Qi, Mengyuan.,Liang, Bin.,Zhang, Long.,Ma, Xiaodan.,Yan, Lei.,...&Wang, Aijie.(2021).Microbial Interactions Drive the Complete Catabolism of the Antibiotic Sulfamethoxazole in Activated Sludge Microbiomes.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(5),3270-3282. |
| MLA | Qi, Mengyuan,et al."Microbial Interactions Drive the Complete Catabolism of the Antibiotic Sulfamethoxazole in Activated Sludge Microbiomes".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.5(2021):3270-3282. |
入库方式： OAI收割
来源：生态环境研究中心
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