Modulatory function of calmodulin on phagocytosis and potential regulation mechanisms in the blood clam Tegillarca granosa
文献类型：期刊论文
| 作者 | Han, Yu; Tang, Yu; Sun, Shuge; Kim, Tongchol; Ju, Kwangjin; Ri, Sanghyok; Du, Xueying; Zhou, Weishang; Shi, Wei; Li, Shiguo |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2021-03 |
| 卷号 | 116页码:- |
| ISSN号 | 0145-305X |
| 关键词 | Calmodulin Phagocytosis Ca2+ signaling pathway Actin cytoskeleton Calcineurin NF-kappa B |
| 英文摘要 | Unlike vertebrate species, invertebrates lack antigen-antibody mediated immune response and mainly rely on haemocyte phagocytosis to fight against pathogen infection. Recently, studies conducted in model vertebrates demonstrated that the multifunctional protein calmodulin (CaM) plays an important role in regulating immune responses. However, the intrinsic relation between CaM and phagocytosis process remains poorly understood in invertebrate species such as bivalve mollusks. Therefore, in the present study, the immunomodulatory function of CaM on haemocyte phagocytosis was verified in the blood clam, Tegillarca granosa, using the CaM-specific inhibitor N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide hydrochloride (W-7). Results obtained show that CaM inhibition significantly suppressed the phagocytic activity of haemocytes. In addition, CaM inhibition constrained intracellular Ca2+ elevation, hampered actin cytoskeleton assembly, suppressed calcineurin (CaN) activity, and disrupted NF-kappa B activation in haemocytes upon LPS induction. Furthermore, expression of seven selected genes from the actin cytoskeleton regulation- and immune-related pathways were significantly down-regulated whereas those of CaM and CaN from the Ca2+-signaling pathway were significantly upregulated by in vitro incubation of haemocytes with W-7. For the first time, the present study demonstrated that CaM play an important role in phagocytosis modulation in bivalve species. In addition, the intracellular Ca2+ and downstream Ca2+ -signaling-, actin cytoskeleton regulation-, and immune-related pathways offer candidate routes through which CaM modulates phagocytosis. |
| WOS研究方向 | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46134]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 作者单位 | 1.Kim Hyong Jik Univ Educ, Coll Life Sci, Pyongyang 99903, South Korea 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Environm Biotechnol, Beijing 100085, Peoples R China 3.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China 4.Wonsan Fisheries Univ, Coll Aquaculture, Wonsan 999093, North Korea |
| 推荐引用方式 GB/T 7714 | Han, Yu,Tang, Yu,Sun, Shuge,et al. Modulatory function of calmodulin on phagocytosis and potential regulation mechanisms in the blood clam Tegillarca granosa[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,116:-. |
| APA | Han, Yu.,Tang, Yu.,Sun, Shuge.,Kim, Tongchol.,Ju, Kwangjin.,...&Liu, Guangxu.(2021).Modulatory function of calmodulin on phagocytosis and potential regulation mechanisms in the blood clam Tegillarca granosa.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,116,-. |
| MLA | Han, Yu,et al."Modulatory function of calmodulin on phagocytosis and potential regulation mechanisms in the blood clam Tegillarca granosa".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 116(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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