Antimicrobial peptide antibiotics inhibit aerobic denitrification via affecting electron transportation and remolding carbon metabolism
文献类型：期刊论文
| 作者 | Wang, Na ; Gao, Jie; Wang, Qiuying; Xiao, Shujie; Zhuang, Guoqiang
|
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2022-06-05 |
| 卷号 | 431期号:0页码:128616 |
| 关键词 | MICROBIAL DENITRIFICATION PARACOCCUS-DENITRIFICANS COMMUNITY STRUCTURE NITRATE TREHALOSE NITROGEN WATER REMOVAL NITRIFICATION PERFORMANCE |
| ISSN号 | 0304-3894 |
| 英文摘要 | The harmful effects of antibiotics on biological denitrification have attracted widespread attention due to their excessive usage. Polymyxin B (PMB) as the typical antimicrobial peptides having been regarded as the last hope for treatment of multidrug-resistance bacteria, has also been detected in wastewater. However, little is known about the influence of PMB on aerobic denitrification. In this study, the impact of PMB on aerobic denitrification performance was investigated. Results showed 0.50 mg/L PMB decreased nitrate removal effi-ciency from 97.4% to 85.3%, and drove denitrifiers to transform more nitrate to biomass instead of producing gas-N. The live/dead staining method showed PMB damaged bacterial membrane. Transcriptome analysis further indicated the key enzymes participating in denitrification and aerobic respiratory chains were suppressed by PMB. To resist the PMB stress, denitrifiers formed thicker biofilm to protect cells from PMB damaging and thus remodeling the central carbon metabolism. Further investigation revealed denitrifiers have different preference on various carbon sources when PMB is present. Subsequently, the underlying mechanism of the distinctive carbon sources preference was explored by the combination of transcriptome and metabolism analysis. Overall, our data suggested denitrifiers have distinctive carbon sources preference under PMB treatment conditions, reminding us that carbon source selection should be cautious in practical applications. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47769]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, CAS Key Lab Environm Biotechnol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Na,Gao, Jie,Wang, Qiuying,et al. Antimicrobial peptide antibiotics inhibit aerobic denitrification via affecting electron transportation and remolding carbon metabolism[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,431(0):128616. |
| APA | Wang, Na,Gao, Jie,Wang, Qiuying,Xiao, Shujie,&Zhuang, Guoqiang.(2022).Antimicrobial peptide antibiotics inhibit aerobic denitrification via affecting electron transportation and remolding carbon metabolism.JOURNAL OF HAZARDOUS MATERIALS,431(0),128616. |
| MLA | Wang, Na,et al."Antimicrobial peptide antibiotics inhibit aerobic denitrification via affecting electron transportation and remolding carbon metabolism".JOURNAL OF HAZARDOUS MATERIALS 431.0(2022):128616. |
入库方式： OAI收割
来源：生态环境研究中心
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