Diffusible signal factor enhances the saline-alkaline resistance and rhizosphere colonization of Stenotrophomonas rhizophila by coordinating optimal metabolism
文献类型：期刊论文
| 作者 | Liu, Ying; Gao, Jie ; Wang, Na; Li, Xianglong; Fang, Na; Zhuang, Xuliang
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2022-08-15 |
| 卷号 | 834期号:0页码:155403 |
| 关键词 | VI SECRETION SYSTEM XANTHOMONAS-CAMPESTRIS BIOFILM FORMATION FATTY-ACIDS DSF-FAMILY STRESS GROWTH EXPRESSION TOLERANCE DOMAIN |
| ISSN号 | 0048-9697 |
| 英文摘要 | Quorum sensing (QS) regulates various physiological processes in a cell density-dependentmode via cell-cell communication. Stenotrophomonas rhizophila DSM14405(T) having the diffusible signal factor (DSF)-QS system, is a plant growth-promoting rhizobacteria (PGPR) that enables host plants to tolerate saline-alkaline stress. However, the regulatory mechanism of DSF-QS in S. rhizophila is not fully understood. In this study, we used S. rhizophila DSM14405(T) wild-type (WT) and an incompetent DSF production rpfF-knockout mutant to explore the regulatory role of QS in S. rhizophila growth, stress responses, biofilm formation, and colonization under saline-alkaline stress. We found that a lack of DSF-QS reduces the tolerance of S. rhizosphere Delta rpfF to saline-alkaline stress, with a nearly 25-fold reduction in the Delta rpfF population compared with WT at 24 h under stress. Transcriptome analysis revealed that QS helps S. rhizophila WT respond to saline-alkaline stress by enhancing metabolism associated with the cell wall and membrane, oxidative stress response, cell adhesion, secretion systems, efflux pumps, and TonB systems. These metabolic systems enhance penetration defense, Na+ efflux, iron uptake, and reactive oxygen species scavenging. Additionally, the absence of DSF-QS causes overexpression of biofilm-associated genes under the regulation of sigma 54 and other transcriptional regulators. However, greater biofilm formation capacity confers no advantage on S. rhizosphere Delta rpfF in rhizosphere colonization. Altogether, our results show the importance of QS in PGPR growth and colonization; QS gives PGPR a collective adaptive advantage in harsh natural environments. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47778]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 通讯作者 | Zhuang, Xuliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Tibetan Plateau Res, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, CAS Key Lab Environm Biotechnol, Beijing 100085, Peoples R China 4.Yunan Univ, Inst Int Rivers & Ecosecur, Kunming 650500, Yunnan, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci Life Sci & Med, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Gao, Jie,Wang, Na,et al. Diffusible signal factor enhances the saline-alkaline resistance and rhizosphere colonization of Stenotrophomonas rhizophila by coordinating optimal metabolism[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,834(0):155403. |
| APA | Liu, Ying,Gao, Jie,Wang, Na,Li, Xianglong,Fang, Na,&Zhuang, Xuliang.(2022).Diffusible signal factor enhances the saline-alkaline resistance and rhizosphere colonization of Stenotrophomonas rhizophila by coordinating optimal metabolism.SCIENCE OF THE TOTAL ENVIRONMENT,834(0),155403. |
| MLA | Liu, Ying,et al."Diffusible signal factor enhances the saline-alkaline resistance and rhizosphere colonization of Stenotrophomonas rhizophila by coordinating optimal metabolism".SCIENCE OF THE TOTAL ENVIRONMENT 834.0(2022):155403. |
入库方式： OAI收割
来源：生态环境研究中心
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