Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes
文献类型：期刊论文
| 作者 | Zheng, Xiaoxuan ; Jahn, Martin T.; Sun, Mingming; Friman, Ville-Petri; Balcazar, Jose Luis; Wang, Jinfeng; Shi, Yu; Gong, Xin; Hu, Feng; Zhu, Yong-Guan
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| 刊名 | ISME JOURNAL
 |
| 出版日期 | 2022-05 |
| 卷号 | 16期号:5页码:1397-1408 |
| 关键词 | ORGANIC POLLUTANTS RESISTANCE GENES PROTEIN BACTERIA ENZYMES DIVERSE BACTERIOPHAGES ECOSYSTEMS PREDICTION ALIGNMENT |
| ISSN号 | 1751-7362 |
| 英文摘要 | Viruses significantly influence local and global biogeochemical cycles and help bacteria to survive in different environments by encoding various auxiliary metabolic genes (AMGs) associated with energy acquisition, stress tolerance and degradation of xenobiotics. Here we studied whether bacterial (dsDNA) virus encoded AMGs are enriched in organochlorine pesticide (OCP) contaminated soil in China and if viral AMGs include genes linked to OCP biodegradation. Using metagenomics, we found that OCP-contaminated soils displayed a lower bacterial, but higher diversity of viruses that harbored a higher relative abundance of AMGs linked to pesticide degradation and metabolism. Furthermore, the diversity and relative abundance of AMGs significantly increased along with the severity of pesticide contamination, and several biodegradation genes were identified bioinformatically in viral metagenomes. Functional assays were conducted to experimentally demonstrate that virus-encoded L-2-haloacid dehalogenase gene (L-DEX) is responsible for the degradation of L-2-haloacid pesticide precursors, improving bacterial growth at sub-inhibitory pesticide concentrations. Taken together, these results demonstrate that virus-encoded AMGs are linked to bacterial metabolism and biodegradation, being more abundant and diverse in soils contaminated with pesticides. Moreover, our findings highlight the importance of virus-encoded accessory genes for bacterial ecology in stressful environments, providing a novel avenue for using viruses in the bioremediation of contaminated soils. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47762]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 作者单位 | 1.Univ Oxford, Dept Chem, Oxford OX1 3SZ, England 2.Jiangsu Collaborat Innovat Ctr Solid Organ Waste, Key Lab Plant Immun, Nanjing 210095, Peoples 3.Nanjing Agr Univ, Coll Resources & Environm Sci, Soil Ecol Lab, Nanjing 210095, Peoples R China 4.Univ Oxford, Dept Biochem, Oxford OX1 3SZ, England 5.Univ Oxford, Dept Zool, Oxford OX1 3SZ, England |
| 推荐引用方式 GB/T 7714 | Zheng, Xiaoxuan,Jahn, Martin T.,Sun, Mingming,et al. Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes[J]. ISME JOURNAL,2022,16(5):1397-1408. |
| APA | Zheng, Xiaoxuan.,Jahn, Martin T..,Sun, Mingming.,Friman, Ville-Petri.,Balcazar, Jose Luis.,...&Zhu, Yong-Guan.(2022).Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes.ISME JOURNAL,16(5),1397-1408. |
| MLA | Zheng, Xiaoxuan,et al."Organochlorine contamination enriches virus-encoded metabolism and pesticide degradation associated auxiliary genes in soil microbiomes".ISME JOURNAL 16.5(2022):1397-1408. |
入库方式： OAI收割
来源：生态环境研究中心
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