Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles
文献类型：期刊论文
| 作者 | Liu Z ; Li M ; Li ZH ; Pu F ; Ren JS ; Qu XG |
| 刊名 | nano research
 |
| 出版日期 | 2012 |
| 卷号 | 5期号:7页码:450-459 |
| 关键词 | MAGNETIC NANOPARTICLES AFFINITY-CHROMATOGRAPHY SHELL SYSTEM NANOCOMPOSITE MICROSPHERES NANOCRYSTALS PURIFICATION PARTICLES NANORODS |
| ISSN号 | 1998-0124 |
| 通讯作者 | ren js |
| 中文摘要 | the development of simple techniques for the separation and purification of recombinant proteins plays an important role in many of the advancements made in biotechnology and nanotechnology. herein, we report an easy method for the efficient purification of polyhistidine affinity-tagged (his-tagged) proteins by using ni2+-decorated superparamagnetic particles. monodisperse ni0.3fe0.7fe2o4 nanoparticles were prepared via a facile and economical one-pot hydrothermal process. owing to the characteristic molecular recognition ability between nickel(ii) ions and the polyhistidine affinity tag, the nanoparticles could be successfully employed to selectively bind and separate his-tagged cyan fluorescent protein (cfp) from an e. coli cell lysate in a recyclable process. moreover, by changing the divalent metal precursors, various other metal-decorated magnetic nanoparticles can be obtained. this approach offers the possibility of constructing metal-decorated nanoparticles through a simple method and will be highly beneficial in further applications of nanoparticle-based technologies. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000306593900002 |
| 公开日期 | 2013-10-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48818]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu Z,Li M,Li ZH,et al. Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles[J]. nano research,2012,5(7):450-459. |
| APA | Liu Z,Li M,Li ZH,Pu F,Ren JS,&Qu XG.(2012).Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles.nano research,5(7),450-459. |
| MLA | Liu Z,et al."Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles".nano research 5.7(2012):450-459. |
入库方式： OAI收割
来源：长春应用化学研究所
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