Carbamazepine at environmentally relevant concentrations caused DNA damage and apoptosis in the liver of Chinese rare minnows (Gobiocypris rarus) by the Ras/Raf/ERK/p53 signaling pathway
文献类型：期刊论文
| 作者 | Yan, Saihong; Chen, Rui; Wang, Miao; Zha, Jinmiao |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2021-02-01 |
| 卷号 | 270页码:- |
| ISSN号 | 0269-7491 |
| 关键词 | Carbamazepine (CBZ) RT-PCR 8-OHdG TUNEL assay Comet assay |
| 英文摘要 | To assess genetoxicity and the underlying mechanisms of carbamazepine ( CBZ) toxicity in fish, adult Chinese rare minnows (Gobiocypris rarus) were exposed to 1, 10, and 100 mu g/L CBZ for 28 d. Comet assays indicated that hepatic DNA damage was significantly increased in groups of minnows exposed to CBZ at all concentrations in a dose-dependent manner compared to those of the control groups (p < 0.05). Liver levels of 8-hydroxydeoxyguanosine (8-OHdG) were significantly increased at 10 and 100 mu g/L CBZ (p < 0.05). TUNEL assays indicated that the average apoptotic rates of the livers of female and male minnows were significantly increased following exposure to CBZ at all concentrations for 28 d (p < 0.05). Significant increases in caspase 3 and 9 activities after CBZ exposure at all concentrations and caspase 8 at 10 and 100 mu g/L CBZ exposure reflected the presence of mitochondrial apoptosis (p < 0.05). The mRNA levels of gadd45a, mdm2, casp3 and casp9 in female and male minnows exposed to CBZ at all concentrations were significantly increased compared with those in the control groups (p < 0.05). Significant increases in the levels of p21 in female minnows exposed to 1 and 100 mu g/L CBZ, p53 in female minnows at all CBZ treatments and bcl2 in male minnows exposed to 1 and 100 mu g/L CBZ were observed, indicating p53 pathway activation. The inhibition of ras levels in females and males exposed to CBZ at all concentrations and increased levels of raf1 in males exposed to CBZ at all concentrations indicated Ras/ Raf1/MAPK (ERK) activation. Therefore, the present study demonstrates that CBZ at environmentally relevant levels induces DNA damage and apoptosis in Chinese rare minnows by the Ras/Raf/ERK/p53 signaling pathway. (C) 2020 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46175]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 作者单位 | 1.China Machinery Int Engn Design & Res Inst, Changsha 410000, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing Key Lab Ind Wastewater Treatment & Reuse, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, 18 Shuangqing Rd, Beijing 100085, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Saihong,Chen, Rui,Wang, Miao,et al. Carbamazepine at environmentally relevant concentrations caused DNA damage and apoptosis in the liver of Chinese rare minnows (Gobiocypris rarus) by the Ras/Raf/ERK/p53 signaling pathway[J]. ENVIRONMENTAL POLLUTION,2021,270:-. |
| APA | Yan, Saihong,Chen, Rui,Wang, Miao,&Zha, Jinmiao.(2021).Carbamazepine at environmentally relevant concentrations caused DNA damage and apoptosis in the liver of Chinese rare minnows (Gobiocypris rarus) by the Ras/Raf/ERK/p53 signaling pathway.ENVIRONMENTAL POLLUTION,270,-. |
| MLA | Yan, Saihong,et al."Carbamazepine at environmentally relevant concentrations caused DNA damage and apoptosis in the liver of Chinese rare minnows (Gobiocypris rarus) by the Ras/Raf/ERK/p53 signaling pathway".ENVIRONMENTAL POLLUTION 270(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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