Comparing the bacterial growth potential of ultra-low nutrient drinking water assessed by growth tests based on flow cytometric intact cell count versus adenosine triphosphate
文献类型：期刊论文
| 作者 | Sousi, Mohaned; Salinas-Rodriguez, Sergio G.; Liu, Gang; Dusseldorp, Jos; Kemperman, Antoine J. B.; Schippers, Jan C.; Van der Meer, Walter G. J.; Kennedy, Maria D. |
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2021-09-15 |
| 卷号 | 203页码:- |
| ISSN号 | 0043-1354 |
| 关键词 | Bacterial growth potential (BGP) Reverse osmosis (RO) Adenosine triphosphate (ATP) Flow cytometry (FCM) Bacterial yield Ultra-low nutrient drinking water |
| 英文摘要 | The bacterial growth potential (BGP) of drinking water is widely assessed either by flow cytometric intact cell count (BGP(ICC)) or adenosine triphosphate (BGP(ATP)) based methods. Combining BGP(ICC) and BGP(ATP) measure-ments has been previously applied for various types of drinking water having high to low growth potential. However, this has not been applied for water with ultra-low nutrient content, such as remineralised RO permeate. To conduct a sound comparison, conventionally treated drinking water was included in this study, which was also used as an inoculum source. BGP(ICC), BGP(ATP), intact cell-yield (Y-ICC), and ATP-yield (Y-ATP) were determined for conventionally treated drinking water (Tap-water) and remineralised RO permeate (RO-water). In addition, both BGP(ICC )and BGP(ATP )methods were used to identify the growth-limiting nutrient in each water type. The results showed that the BGP(ICC) ratio between Tap-water/RO-water was similar to 7.5, whereas the BGP(ATP) ratio was only similar to 4.5. Moreover, the Y-ICC ratio between Tap-water/RO-water was similar to 2 (9.8 +/- 0.6 x 10(6) vs. 4.6 +/- 0.8 x 10(6) cells/mu g-C), whereas the Y-ATP ratio was similar to 1 (0.39 +/- 0.12 vs. 0.42 +/- 0.06 ng ATP/mu g-C), resulting in a consistently higher ATP per cell in RO-water than that of Tap-water. Both BGP(ICC) and BGP(ATP) methods revealed that carbon was the growth-limiting nutrient in the two types of water. However, with the addition of extra carbon, phosphate limitation was detected only with the BGP(ICC) method, whereas BGP(ATP )was not affected, suggesting that a combination of carbon and phosphate is essential for biomass synthesis, whereas carbon is probably utilised for cellular activities other than cell synthesis when phosphate is limited. It was estimated that the intact cell-yield growing on phosphate would be 0.70 +/- 0.05 x 10(9) cells/mu g PO4-P. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences ; Water Resources |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46182]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 作者单位 | 1.IHE Delft Inst Water Educ Sanitat & Environm Engn, Dept Water Supply, Westvest 7, NL-2611 AX Delft, Netherlands 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 3.Univ Twente, Fac Sci & Technol, Drienerlolaan 5, NL-7522 NB Enschede, Netherlands 4.Oasen Drinkwater, Nieuwe Gouwe OZ 3, NL-2801 SB Gouda, Netherlands 5.Delft Univ Technol, Fac Civil Engn & Geosci, Dept Water Management, Mekelweg 2, NL-2628 CD Delft, Netherlands |
| 推荐引用方式 GB/T 7714 | Sousi, Mohaned,Salinas-Rodriguez, Sergio G.,Liu, Gang,et al. Comparing the bacterial growth potential of ultra-low nutrient drinking water assessed by growth tests based on flow cytometric intact cell count versus adenosine triphosphate[J]. WATER RESEARCH,2021,203:-. |
| APA | Sousi, Mohaned.,Salinas-Rodriguez, Sergio G..,Liu, Gang.,Dusseldorp, Jos.,Kemperman, Antoine J. B..,...&Kennedy, Maria D..(2021).Comparing the bacterial growth potential of ultra-low nutrient drinking water assessed by growth tests based on flow cytometric intact cell count versus adenosine triphosphate.WATER RESEARCH,203,-. |
| MLA | Sousi, Mohaned,et al."Comparing the bacterial growth potential of ultra-low nutrient drinking water assessed by growth tests based on flow cytometric intact cell count versus adenosine triphosphate".WATER RESEARCH 203(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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