PEGylation of bovine serum albumin using click chemistry for the application as drug carriers
文献类型：期刊论文
| 作者 | Li XY ; Li TH ; Guo JS ; Wei Y ; Jing XB ; Chen XS ; Huang YB |
| 刊名 | biotechnology progress
 |
| 出版日期 | 2012 |
| 卷号 | 28期号:3页码:856-861 |
| 关键词 | COVALENT ATTACHMENT POLYETHYLENE-GLYCOL PARTICLE-SIZE IN-VITRO POLYMER RELEASE CHEMOTHERAPY PROTEINS PEG BIOCONJUGATION |
| ISSN号 | 8756-7938 |
| 通讯作者 | huang yb |
| 中文摘要 | monomethyl poly(ethylene glycol) (mpeg)-modified bovine serum albumin (bsa) conjugates (bsa-mpeg) were obtained by the mild cu(i)-mediated cycloaddition reaction of azided bsa (bsa-n3) and alkyne-terminated mpeg. the structure and characteristics of bsa-mpeg conjugates were thoroughly investigated. there were about two peg chains conjugated onto each bsa molecule as determined by matrix-assisted laser desorption/ionization time of flight (maldi-tof) analysis. the intrinsic nonspecific binding ability of bsa was used for adsorption and sustained release of both rifampicn and 5-fluorouracil (5-fu). the helical structures of bsa were preserved to a large extent after modification and drug adsorption on bsa was confirmed via circular dichroism spectroscopy. drugs adsorbed onto the conjugated formulation to a lesser extent than on bsa due to mpeg modification. the in vitro release of both rifampicin and 5-fu, however, indicated that bsa-mpeg can function as a drug carrier. overall, the click reaction provided a convenient tool for the pegylation of bsa. the biological activity of the bsa-mpeg conjugates, including the drug transportation capacity and biocompatibility, were largely retained. (c) 2012 american institute of chemical engineers biotechnol. prog.,, 2012 |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000304989800026 |
| 公开日期 | 2013-10-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48829]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li XY,Li TH,Guo JS,et al. PEGylation of bovine serum albumin using click chemistry for the application as drug carriers[J]. biotechnology progress,2012,28(3):856-861. |
| APA | Li XY.,Li TH.,Guo JS.,Wei Y.,Jing XB.,...&Huang YB.(2012).PEGylation of bovine serum albumin using click chemistry for the application as drug carriers.biotechnology progress,28(3),856-861. |
| MLA | Li XY,et al."PEGylation of bovine serum albumin using click chemistry for the application as drug carriers".biotechnology progress 28.3(2012):856-861. |
入库方式： OAI收割
来源：长春应用化学研究所
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