Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)
文献类型：期刊论文
| 作者 | Zhang, Dayi; Zhang, Xiaoling; Ma, Rui; Deng, Songqiang; Wang, Xinzi; Wang, Xinquan; Zhang, Xian; Huang, Xia; Liu, Yi; Li, Guanghe |
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2021-07-15 |
| 卷号 | 200页码:- |
| ISSN号 | 0043-1354 |
| 关键词 | SARS-CoV-2 Surface enhanced Raman scattering (SERS) Human receptor angiotensin converting enzyme 2 (ACE2) Wastewater-based epidemiology |
| 英文摘要 | The outbreak of coronavirus infectious disease-2019 (COVID-19) pneumonia challenges the rapid interrogation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human and environmental samples. In this study, we developed an assay using surface enhanced Raman scattering (SERS) coupled with multivariate analysis to detect SARS-CoV-2 in an ultra-fast manner without any pretreatment (e.g., RNA extraction). Using silver-nanorod SERS array functionalized with cellular receptor angiotensin-converting enzyme 2 (ACE2), we obtained strong SERS signals of ACE2 at 1032, 1051, 1089, 1189, 1447 and 1527 cm(-1). The recognition and binding of receptor binding domain (RBD) of SARS-CoV-2 spike protein on SERS assay significantly quenched the spectral intensities of most peaks and exhibited a shift from 1189 to 1182 cm(-1). On-site tests on 23 water samples with a portable Raman spectrometer proved its accuracy and easy-operation for spot detection of SARS-CoV-2 to evaluate disinfection performance, explore viral survival in environmental media, assess viral decay in wastewater treatment plant and track SARS-CoV-2 in pipe network. Our findings raise a state-of-the-art spectroscopic tool to screen and interrogate viruses with RBD for human cell entry, proving its feasibility and potential as an ultra-fast detection tool for wastewater-based epidemiology. (C) 2021 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences ; Water Resources |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/46272]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 作者单位 | 1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 3.Suzhou Yiqing Environm Sci & Technol LTD, Suzhou 215163, Peoples R China 4.Res Inst Environm Innovat Tsinghua Suzhou, Suzhou 215163, Peoples R China 5.Chinese Acad Sci, Suzhou Inst Nanotech & Nanob, 398 Ruoshui Rd,Suzhou Ind Pk, Suzhou 215 6.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dayi,Zhang, Xiaoling,Ma, Rui,et al. Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)[J]. WATER RESEARCH,2021,200:-. |
| APA | Zhang, Dayi.,Zhang, Xiaoling.,Ma, Rui.,Deng, Songqiang.,Wang, Xinzi.,...&Li, Junyi.(2021).Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS).WATER RESEARCH,200,-. |
| MLA | Zhang, Dayi,et al."Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)".WATER RESEARCH 200(2021):-. |
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来源：生态环境研究中心
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