The Interactions of Spore-Coat Morphogenetic Proteins Studied by Single-Molecule Recognition Force Spectroscopy
文献类型：期刊论文
| 作者 | Qiao HY ; Krajcikova D ; Liu CS ; Li YJ ; Wang HD ; Barak I ; Tang JL |
| 刊名 | chemistry-an asian journal
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4页码:725-731 |
| 关键词 | BACILLUS-SUBTILIS SPORE BACTERIAL S-LAYER AFFINITY-TAGS MICROSCOPY RESISTANCE MECHANISMS COTE LOCALIZATION PATTERNS SURFACE |
| ISSN号 | 1861-4728 |
| 通讯作者 | tang jl |
| 中文摘要 | bacillus subtilis can form a spore, which is a dormant type of cell, when its external environment becomes unsuitable for vegetative growth. the spore is surrounded by a multilayered proteinaceous shell called a spore coat, which plays a crucial role in dormancy and germination. of the over 70 proteins that form the spore coat, only a small subset of them affect its morphogenesis; they are referred to as morphogenetic proteins. how these morphogenetic proteins interact, and furthermore, how they build the ordered, functional coat layers is not well understood. elucidating the self-assembly mechanism of individual proteins into such a complex structure may contribute to its potential use in nano-biotechnology applications for preparing highly organized, robust, and resistant proteinaceous layers. herein, direct, noncovalent, low-affinity interactions between the spore-coat morphogenetic proteins spoiva, spovid, and safa were studied by using single-molecule recognition force spectroscopy in vitro for the first time. based on the real-time examination of interactions between these three proteins, a series of dynamic kinetic data were obtained. it was also observed that the safaspovid interaction was stronger than that of safaspoiva. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302159900015 |
| 公开日期 | 2013-10-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48841]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qiao HY,Krajcikova D,Liu CS,et al. The Interactions of Spore-Coat Morphogenetic Proteins Studied by Single-Molecule Recognition Force Spectroscopy[J]. chemistry-an asian journal,2012,7(4):725-731. |
| APA | Qiao HY.,Krajcikova D.,Liu CS.,Li YJ.,Wang HD.,...&Tang JL.(2012).The Interactions of Spore-Coat Morphogenetic Proteins Studied by Single-Molecule Recognition Force Spectroscopy.chemistry-an asian journal,7(4),725-731. |
| MLA | Qiao HY,et al."The Interactions of Spore-Coat Morphogenetic Proteins Studied by Single-Molecule Recognition Force Spectroscopy".chemistry-an asian journal 7.4(2012):725-731. |
入库方式： OAI收割
来源：长春应用化学研究所
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