Nongenomic effects and mechanistic study of butyl benzyl phthalate-induced thyroid disruption: Based on integrated in vitro, in silico assays and proteome analysis
文献类型：期刊论文
| 作者 | Li, Jian ; Xu, Ying; Jiang, Yuhao; Li, Na; Li, Zhanjie; Kong, Dongdong; Guo, Xueru; Zhang, Junbo; Zuo, Rui
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2022-08-25 |
| 卷号 | 836期号:0页码:155715 |
| ISSN号 | 0048-9697 |
| 关键词 | HORMONE CELLS RECEPTOR ALPHA(V)BETA(3) PROLIFERATION ACTIVATION TOXICITY EXPOSURE GROWTH ESTERS |
| 英文摘要 | Based on in vitro and in silico assays as well as proteome analysis, this study explored the nongcnomic mechanism for butyl benzyl phthalate (BBP)-induced thyroid disruption. Molecular docking simulations showed that BBP could dock into the Arg-Gly-Asp (RGD) domain of intcgrin alpha(v)beta(3) and form hydrogen bonds with a docking energy of -35.80 kcal/ mol. This chemical enhanced rat pituitary tumor cell (GH3) proliferation and exhibited thyroid hormone-disrupting effects at 5-10 mu mol/L. Meanwhile, BBP upregulated beta(3) gene expression and activated the downstream mitogcnactivated protein kinase (MAPK) pathway in GH3 cells. Interestingly, GH3 cell proliferation was attenuated by intcgrin alpha(v)beta(3) inhibitor (RGD peptide) or ERK1/2 inhibitor (PD98059), suggesting that the disruptions might be partly attributed to its interaction with integrin alpha(v)beta(3 )and activation of MAPK. Furthermore, quantitative proteomic analysis of zebrafish embryos exposed to BBP at an environmentally relevant concentration of 0.3 mu mol/L revealed that BBP perturbed proteins and pathways related to cell communication (e.g., integrin binding) and signal transduction (e.g., MAPK signaling pathway). Taken together, our results supported that the biological effects of BBP-activated integrin alpha(v)beta(3) mediated by the nongenomic pathway play an important role in its thyroid disruption. Capsule: The nongenomic pathway plays a vital role in the thyroid disruption-inducing actions of BBP. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47881]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 通讯作者 | Zuo, Rui |
| 作者单位 | 1.Beijing Normal Univ, Coll Chem, Key Lab Radiopharmaceut, Minist Educ, Beijing 100875, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 3.Beijing Normal Univ, Coll Water Sci, Engn Res Ctr Groundwater Pollut Control & Remedia, Minist Educ, Beijing 100875, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jian,Xu, Ying,Jiang, Yuhao,et al. Nongenomic effects and mechanistic study of butyl benzyl phthalate-induced thyroid disruption: Based on integrated in vitro, in silico assays and proteome analysis[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,836(0):155715. |
| APA | Li, Jian.,Xu, Ying.,Jiang, Yuhao.,Li, Na.,Li, Zhanjie.,...&Zuo, Rui.(2022).Nongenomic effects and mechanistic study of butyl benzyl phthalate-induced thyroid disruption: Based on integrated in vitro, in silico assays and proteome analysis.SCIENCE OF THE TOTAL ENVIRONMENT,836(0),155715. |
| MLA | Li, Jian,et al."Nongenomic effects and mechanistic study of butyl benzyl phthalate-induced thyroid disruption: Based on integrated in vitro, in silico assays and proteome analysis".SCIENCE OF THE TOTAL ENVIRONMENT 836.0(2022):155715. |
入库方式： OAI收割
来源：生态环境研究中心
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