Phthalate Esters Released from Plastics Promote Biofilm Formation and Chlorine Resistance
文献类型：期刊论文
| 作者 | Wang, Haibo ; Yu, Pingfeng; Schwarz, Cory; Zhang, Bo; Huo, Lixin; Shi, Baoyou; Alvarez, Pedro J. J.
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2022-01-18 |
| 卷号 | 56期号:2页码:1081-1090 |
| ISSN号 | 0013-936X |
| 关键词 | EXTRACELLULAR POLYMERIC SUBSTANCES DRINKING-WATER MICROBIOME PSEUDOMONAS-AERUGINOSA RISK-ASSESSMENT GENE-EXPRESSION MONOCHLORAMINE DISINFECTION MECHANISMS DETACHMENT BACTERIA |
| 英文摘要 | Phthalate esters (PAEs) are commonly released from plastic pipes in some water distribution systems. Here, we show that exposure to a low concentration (1-10 mu g/L) of three PAEs (dimethyl phthalate (DMP), di-n-hexyl phthalate (DnHP), and di-(2-ethylhexyl) phthalate (DEHP)) promotes Pseudomonas biofilm formation and resistance to free chlorine. At PAE concentrations ranging from 1 to 5 mu g/L, genes coding for quorum sensing, extracellular polymeric substances excretion, and oxidative stress resistance were upregulated by 2.7- to 16.8-fold, 2.1- to 18.9-fold, and 1.6- to 9.9-fold, respectively. Accordingly, more biofilm matrix was produced and the polysaccharide and eDNA contents increased by 30.3-82.3 and 10.3-39.3%, respectively, relative to the unexposed controls. Confocal laser scanning microscopy showed that PAE exposure stimulated biofilm densification (volumetric fraction increased from 27.1 to 38.0-50.6%), which would hinder disinfectant diffusion. Biofilm densification was verified by atomic force microscopy, which measured an increase of elastic modulus by 2.0- to 3.2-fold. PAE exposure also stimulated the antioxidative system, with cell-normalized superoxide dismutase, catalase, and glutathione activities increasing by 1.8- to 3.0-fold, 1.0- to 2.0-fold, and 1.2- to 1.6-fold, respectively. This likely protected cells against oxidative damage by chlorine. Overall, we demonstrate that biofilm exposure to environmentally relevant levels of PAEs can upregulate molecular processes and physiologic changes that promote biofilm densification and antioxidative system expression, which enhance biofilm resistance to disinfectants. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47892]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 作者单位 | 1.Northeast Agr Univ, Sch Resources & Environm, Harbin 150030, Peoples R China 2.Rice Univ, Dept Civil & Environm Engn, Houston, TX 77005 USA 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 4.Zhejiang Univ, Coll Environm & Resource Sci, Hangzhou 310058, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Haibo,Yu, Pingfeng,Schwarz, Cory,et al. Phthalate Esters Released from Plastics Promote Biofilm Formation and Chlorine Resistance[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(2):1081-1090. |
| APA | Wang, Haibo.,Yu, Pingfeng.,Schwarz, Cory.,Zhang, Bo.,Huo, Lixin.,...&Alvarez, Pedro J. J..(2022).Phthalate Esters Released from Plastics Promote Biofilm Formation and Chlorine Resistance.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(2),1081-1090. |
| MLA | Wang, Haibo,et al."Phthalate Esters Released from Plastics Promote Biofilm Formation and Chlorine Resistance".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.2(2022):1081-1090. |
入库方式： OAI收割
来源：生态环境研究中心
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