Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies
文献类型：期刊论文
| 作者 | Li, Jian ; Xu, Ying; Li, Na; Zuo, Rui; Zhai, Yuanzheng; Chen, Haiyang
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2022-04-05 |
| 卷号 | 56期号:7页码:4241-4250 |
| ISSN号 | 0013-936X |
| 关键词 | FLAME RETARDANTS INTEGRIN ALPHA(V)BETA(3) TRIPHENYL PHOSPHATE EXPOSURE ALPHA-V-BETA-3 EMBRYOS/LARVAE ACTIVATION TOXICITY ANIMALS TDCPP |
| 英文摘要 | Earlier mechanistic studies of many prohibited flame retardants (FRs) highlighted their thyroid hormone-disrupting activity through nuclear thyroid hormone receptors (nTRs), whereas some alternative FRs such as organophosphate esters (OPEs) exerted weak nTR-disrupting effects. However, an increasing number of studies have revealed that OPEs also exert thyroid hormone-disrupting effects, and the underlying mechanism is unclear. Herein, the thyroid hormone-disrupting effects and mechanisms of 8 typical OPEs were investigated using integrated in vitro, in vivo, and in silico assays. All tested chemicals competitively bound to the membrane thyroid hormone receptor (mTR) [the 20% relative inhibitory concentration (RIC20): (3.5 +/- 0.2) X 10(7) to (4.9 +/- 1.0) X 10(7) nM], and CI-OPEs and alkyl-OPEs had lower RIC20 values. In contrast, only 4 OPEs showed nTR antagonistic activities at higher concentrations [>= (4.8 +/- 0.8) X 10(3) nM]. CI-OPEs and alkyl-OPEs preferentially interacted with mTR. Molecular docking illustrated that OPEs docked into mTRs, consistent with the competitive binding assay. In vivo analyses of zebrafish embryonic development confirmed that tris(1,3-dichloro-2-propyl) phosphate induced inappropriate expression of proteins, and these protein interactions might be associated with mTR according to the quantitative proteomic analysis. Based on the results, mTR might play a critical role in mediating the thyroid hormone-disrupting effects of OPEs. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47910]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 通讯作者 | Li, Jian |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 2.Beijing Normal Univ, Coll Water Sci, Engn Res Ctr Groundwater Pollut Control & Remedia, Minist Educ, Beijing 100875, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jian,Xu, Ying,Li, Na,et al. Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(7):4241-4250. |
| APA | Li, Jian,Xu, Ying,Li, Na,Zuo, Rui,Zhai, Yuanzheng,&Chen, Haiyang.(2022).Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(7),4241-4250. |
| MLA | Li, Jian,et al."Thyroid Hormone Disruption by Organophosphate Esters Is Mediated by Nuclear/Membrane Thyroid Hormone Receptors: In Vitro, In Vivo, and In Silico Studies".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.7(2022):4241-4250. |
入库方式： OAI收割
来源：生态环境研究中心
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