Screen and Verification of Interactive Protein of Selenoprotein K in Human Liver
文献类型：期刊论文
| 作者 | Liang XY ; Liu Q ; Chen P ; Huo KK ; Hu TY ; Ni JZ |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:2页码:313-320 |
| 关键词 | RESPONSE ELEMENT LUMAN TRANSCRIPTION GPX |
| ISSN号 | 0251-0790 |
| 通讯作者 | liu q |
| 中文摘要 | selenium, an essential trace element for human health, plays important roles in maintaining redox balance in vivo. selenium deficiency is associated with many diseases, such as cancer, neurodegenerative diseases and cardiovascular diseases. the biological function of selenium in vivo is mainly exerted through selenoproteins. selenoprotein k(selk) is a newly discovered selenoprotein with unknown biological function and molecular mechanism. in this work, human selk gene was cloned, site-directedly mutated and inserted into the "bait" plasmid to screen the human liver cdna library using the yeast two-hybrid system. an interactive protein, homo sapiens camp responsive element binding protein 3 (creb3), was screened out and analyzed. selk' and creb3 genes were then co-transformed into yeast cells to verify the protein interaction, followed by the co-transfection into hek293t cells to further verify the interaction via three methods of fluorescence resonance energy transfer technique, including the receptor photobleaching, sensitized emission and fluorescence lifetime. the results show that selk interacte with creb3 independent of its selenocysteine residue. by interacting with creb3, selk may participate in creb3-mediated endoplasmic reticulum-associated degradation and make impact on cancer migration and development. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000301023400018 |
| 公开日期 | 2013-10-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48869]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liang XY,Liu Q,Chen P,et al. Screen and Verification of Interactive Protein of Selenoprotein K in Human Liver[J]. chemical journal of chinese universities-chinese,2012,33(2):313-320. |
| APA | Liang XY,Liu Q,Chen P,Huo KK,Hu TY,&Ni JZ.(2012).Screen and Verification of Interactive Protein of Selenoprotein K in Human Liver.chemical journal of chinese universities-chinese,33(2),313-320. |
| MLA | Liang XY,et al."Screen and Verification of Interactive Protein of Selenoprotein K in Human Liver".chemical journal of chinese universities-chinese 33.2(2012):313-320. |
入库方式： OAI收割
来源：长春应用化学研究所
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