Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant
文献类型：期刊论文
| 作者 | Liu, Bo-Ling; Yang, Xia; Liu, Jing; Dong, Yang ; Wang, Yin-Zheng
|
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2014 |
| 卷号 | 118期号:2页码:357-371 |
| 关键词 | Chirita pumila Cleistogamy Evo-devo Genetic transformation Gesneriaceae |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-014-0488-2 |
| 文献子类 | Article |
| 英文摘要 | An efficient transformation and regeneration system is essential for functional investigation of developmental genes and related elements in the field of evolutionary developmental biology (evo-devo). Chirita pumila D. Don belongs to the Gesneriaceae family, one of the most basal groups in Lamiales sensu lato, and possesses many tractable biological features including annual habit, small plant size, short generation time, abundant offspring and low chromosome number. In addition, C. pumila has cleistogamous flowers with potential cross-pollination, a special phenomenon first reported herein in Gesneriaceae. Parameters affecting shoot induction and genetic transformation have been evaluated, including plant growth regulators, temperature, antibiotic concentration, pre- and co-culture duration, Agrobacterium cell density and infection time. Polymerase chain reaction and beta-Glucuronidase (GUS) activity assays of T0 and T1 plants show that the GUS gene has been introduced into the host with stable and universal expression. The applicability of the transformation system in gene function investigation is further confirmed by transforming a GsNST1B gene from Glycine soja. This transformation system provides a valuable platform for deep function analyses of related genes and elements for a wide range of evo-devo studies, especially in the field of floral evolution, which would develop its potential of being a model organism in Lamiales s. l. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5044 |
| WOS关键词 | AGROBACTERIUM-MEDIATED TRANSFORMATION ; EVOLUTIONARY DEVELOPMENTAL BIOLOGY ; CYC-LIKE GENES ; FLOWER SYMMETRY ; HETEROTRICHA GESNERIACEAE ; EXPRESSION PATTERNS ; FLORAL ZYGOMORPHY ; CLADE GENES ; L. ; ARABIDOPSIS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000339279500015 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China [30990240, 31170198] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27183]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.[Liu, Bo-Ling 2.Yang, Xia 3.Liu, Jing 4.Dong, Yang 5.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China 6.Qufu Normal Univ, Qufu, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Bo-Ling,Yang, Xia,Liu, Jing,et al. Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2014,118(2):357-371. |
| APA | Liu, Bo-Ling,Yang, Xia,Liu, Jing,Dong, Yang,&Wang, Yin-Zheng.(2014).Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant.PLANT CELL TISSUE AND ORGAN CULTURE,118(2),357-371. |
| MLA | Liu, Bo-Ling,et al."Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant".PLANT CELL TISSUE AND ORGAN CULTURE 118.2(2014):357-371. |
入库方式： OAI收割
来源：植物研究所
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