Targeting MAGO proteins with a peptide aptamer reinforces their essential roles in multiple rice developmental pathways
文献类型：期刊论文
| 作者 | Gong, Pichang; Quan, Hui1; He, Chaoying
|
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2014 |
| 卷号 | 80期号:5页码:905-914 |
| 关键词 | peptide aptamer male fertility exon junction complex MAGO NASHI (MAGO) Oryza sativa L technical advance |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.12672 |
| 文献子类 | Article |
| 英文摘要 | Peptide aptamers are artificial short peptides that potentially interfere with the biological roles of their target proteins; however, this technology has not yet been applied to plant functional genomics. MAGO and Y14, the two core subunits of the exon junction complex (EJC), form obligate heterodimers in eukaryotes. In Oryza sativa L. (rice), each of the two genes has two homologs, designated OsMAGO1 and OsMAGO2, and OsY14a and OsY14b, respectively. Here, we characterized a 16-amino acida peptide aptamer (PAP) for the rice MAGO proteins. PAP and rice Y14 bound competitively to rice MAGO proteins. Specifically targeting the MAGO proteins by expressing the aptamer in transgenic rice plants did not affect the endogenous synthesis and accumulation of MAGO proteins; however, the phenotypic variations observed in multiple organs phenocopied those of transgenic rice plants harboring RNA interference (RNAi) constructs in which the accumulation of MAGO and/or OsY14a transcripts and MAGO proteins was downregulated severely. Morphologically, the aptamer transgenic plants were short with abnormally developed flowers, and the stamens exhibited reduced degradation and absorption of both the endothecium and tapetum, thus confirming that EJC core heterodimers play essential roles in rice development, growth and reproduction. This study reveals that as a complementary approach of RNAi, peptide aptamers are powerful tools for interfering with the function of proteins in higher plants. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | BIMOLECULAR FLUORESCENCE COMPLEMENTATION ; FUNCTIONAL GENOMICS ; PLANT-CELLS ; DROSOPHILA ; NASHI ; ARABIDOPSIS ; GENE ; REPLICATION ; RESISTANCE ; BIND |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000345511500014 |
| 出版者 | WILEY |
| 资助机构 | Chinese Ministry of Agriculture Transgenic Major Project [2009ZX08009-011B] ; National Natural Science Foundation of China [31370259] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27299]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Pichang,Quan, Hui,He, Chaoying. Targeting MAGO proteins with a peptide aptamer reinforces their essential roles in multiple rice developmental pathways[J]. PLANT JOURNAL,2014,80(5):905-914. |
| APA | Gong, Pichang,Quan, Hui,&He, Chaoying.(2014).Targeting MAGO proteins with a peptide aptamer reinforces their essential roles in multiple rice developmental pathways.PLANT JOURNAL,80(5),905-914. |
| MLA | Gong, Pichang,et al."Targeting MAGO proteins with a peptide aptamer reinforces their essential roles in multiple rice developmental pathways".PLANT JOURNAL 80.5(2014):905-914. |
入库方式： OAI收割
来源：植物研究所
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