A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo
文献类型：期刊论文
| 作者 | Jia, Husen; Dwyer, Simon A.; Fan, Da-Yong; Han, Yaqin; Badger, Murray R.; von Caemmerer, Susanne; Chow, Wah Soon6 |
| 刊名 | PHYSIOLOGIA PLANTARUM
 |
| 出版日期 | 2014 |
| 卷号 | 152期号:3页码:403-413 |
| ISSN号 | 0031-9317 |
| DOI | 10.1111/ppl.12235 |
| 文献子类 | Article |
| 英文摘要 | We sought a rapid, non-intrusive, whole-tissue measure of the functional photosystem II (PS II) content in leaves. Summation of electrons, delivered by a single-turnover flash to P700(+) (oxidized PS I primary donor) in continuous background far-red light, gave a parameter S in absorbance units after taking into account an experimentally determined basal electron flux that affects P700 redox kinetics. S was linearly correlated with the functional PS II content measured by the O-2 yield per single-turnover repetitive flash in Arabidopsis thaliana expressing an antisense construct to the PsbO (manganese-stabilizing protein in PS II) proteins of PS II (PsbO mutants). The ratio of S to z(max) (total PS I content in absorbance units) was comparable to the PS II/PS I reaction-center ratio in wild-type A. thaliana and in control Spinacea oleracea. Both S and S/z(max) decreased in photoinhibited spinach leaf discs. The whole-tissue functional PS II content and the PS II/photosystem I (PS I) ratio can be non-intrusively monitored by S and S/z(max), respectively, using a quick P700 absorbance protocol compatible with modern P700 instruments. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1399-3054 |
| WOS关键词 | PHOTOSYNTHETIC ELECTRON-TRANSPORT ; ABSORBENCY CHANGES ; CHLOROPHYLL FLUORESCENCE ; STROMAL COMPONENTS ; INTACT LEAVES ; QUANTUM YIELD ; CHLOROPLASTS ; PHOTOINHIBITION ; FLASH ; STATE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000343972500001 |
| 出版者 | WILEY |
| 资助机构 | Australian Postgraduate Award ; Chinese Academy of Sciences [KZCX2-XB3-09-02] ; Chinese Academy of Sciences (NNSF of China) [31370424] ; Australian Research Council [DP1093827, CE056159] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27201]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.[Jia, Husen 2.Dwyer, Simon A. 3.Fan, Da-Yong 4.Han, Yaqin 5.Badger, Murray R. 6.von Caemmerer, Susanne 7.Australian Natl Univ, Coll Med Biol & Environm, Res Sch Biol, Div Plant Sci, Canberra, ACT, Australia 8.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Husen,Dwyer, Simon A.,Fan, Da-Yong,et al. A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo[J]. PHYSIOLOGIA PLANTARUM,2014,152(3):403-413. |
| APA | Jia, Husen.,Dwyer, Simon A..,Fan, Da-Yong.,Han, Yaqin.,Badger, Murray R..,...&Chow, Wah Soon.(2014).A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo.PHYSIOLOGIA PLANTARUM,152(3),403-413. |
| MLA | Jia, Husen,et al."A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo".PHYSIOLOGIA PLANTARUM 152.3(2014):403-413. |
入库方式： OAI收割
来源：植物研究所
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