Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea
文献类型：期刊论文
| 作者 | Cao, Di; Stewart, C. Neal, Jr.; Zheng, Min; Guan, Zhengjun; Tang, Zhi-xi; Wei, Wei; Ma, Ke-ping
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| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2014 |
| 卷号 | 227页码:45-50 |
| 关键词 | Brassica napus Bt Cry1Ac protein Gene flow Insect bioassay Introgression Segregation ratio |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2014.06.018 |
| 文献子类 | Article |
| 英文摘要 | Transgenic canola (Brassica napus) with a Bacillus thuringiensis cry1Ac gene and a green fluorescent protein (GFP) marker gene was used in hybridization experiments with wild Brassica juncea. Hybrid F-1 and successive five backcross generations were obtained. The pod-set frequency on backcrossed B. juncea plants was over 66%, which suggested relatively high crossing compatibility between the hybrids and wild species. The seed setting in BC1 was the least of all generations tested, and then increased at the BC2 generation for which the thousand-seed weight was the highest of all generations. Seed size in backcrossed generations eventually approached that of the wild parent. The plants in all backcrossed generations were consistent with the expected 1:1 segregation ratio of the transgenes. The Bt Cry1Ac protein concentrations at bolting and flowering stages was higher compared to the 4-5-leaf and pod-formation stages. Nonetheless, the Bt toxin in the fifth backcrossing generation (BC5) was sufficient to kill both polyphagous (Helicoverpa armigera) and oligophagous (Plutella xylostella) Lepidoptera. As a consequence, the subsequent generations harboring the transgene from F-1 to BC5 could have selection advantage against insect pests. The result is useful in understanding gene flow from transgenic crops and the followed transgene introgression into wild. (C) 2014 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CLARE |
| WOS关键词 | GREEN-FLUORESCENT PROTEIN ; OILSEED RAPE ; HERBICIDE RESISTANCE ; BT TRANSGENES ; HYBRIDS ; EXPRESSION ; HYBRIDIZATION ; FITNESS ; POPULATIONS ; BACKCROSS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000342865000006 |
| 出版者 | ELSEVIER IRELAND LTD |
| 资助机构 | National Natural Science Foundation of China [31270578, 31200422, 31370357] ; Commonweal Scientific Program of Environmental Protection [201109028] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27221]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.[Cao, Di 2.Zheng, Min 3.Guan, Zhengjun 4.Tang, Zhi-xi 5.Wei, Wei 6.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 7.[Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA |
| 推荐引用方式 GB/T 7714 | Cao, Di,Stewart, C. Neal, Jr.,Zheng, Min,et al. Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea[J]. PLANT SCIENCE,2014,227:45-50. |
| APA | Cao, Di.,Stewart, C. Neal, Jr..,Zheng, Min.,Guan, Zhengjun.,Tang, Zhi-xi.,...&Ma, Ke-ping.(2014).Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea.PLANT SCIENCE,227,45-50. |
| MLA | Cao, Di,et al."Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea".PLANT SCIENCE 227(2014):45-50. |
入库方式： OAI收割
来源：植物研究所
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