Cloning of a novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum
文献类型：期刊论文
| 作者 | Zhang, Hong; Guo, Yan-Wu; Yang, Ya-Dong; Ma, Yi-Jiao; Liu, Ben-Ye; Ye, He-Chun; Wang, Hong5; Ma, Lan-Qing |
| 刊名 | JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 23期号:1页码:104-111 |
| 关键词 | Intron Polygonum cuspidatum Polyketide synthase (PKS) |
| ISSN号 | 0971-7811 |
| DOI | 10.1007/s13562-012-0184-4 |
| 文献子类 | Article |
| 英文摘要 | By using the TAIL-PCR and over-hang extension PCR methods, we successfully isolated a three-intron type III PKS gene and cDNA clone from the Polygonum cuspidatum Sieb. et Zucc, and named it PcPKS4. The phylogenetic analysis showed that PcPKS4 is located in a cluster containing all functionally divergent CHS-like enzymes from angiosperms. Interestingly, the CHS gatekeeper phenylalanine, Phe265 is uniquely replaced by Leu in PcPKS4. Different from our previously results, RNA gel-blot analysis revealed that the PcPKS4 transcripts were present at a low level in roots. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | NEW DELHI |
| 电子版国际标准刊号 | 0974-1275 |
| WOS关键词 | SITE-DIRECTED MUTAGENESIS ; CHALCONE SYNTHASE ; BENZALACETONE SYNTHASE ; BIOSYNTHESIS ; ELEMENTS ; FAMILY ; SEQUENCES ; PROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000329619700011 |
| 出版者 | SPRINGER INDIA |
| 资助机构 | National Natural Science Foundation of China [31140068] ; Key Natural Science Foundation of Beijing Municipality [5111001] ; Foundation of Beijing Municipal Education Committee [KM201110020001] ; Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality [PHR201108279] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27039]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.[Zhang, Hong 2.Yang, Ya-Dong 3.Ma, Yi-Jiao 4.China Agr Univ, Minist Agr Peoples Republ China, Key Lab Urban Agr North, Beijing 102206, Peoples R China 5.[Guo, Yan-Wu 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.[Liu, Ben-Ye 8.Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Environm Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hong,Guo, Yan-Wu,Yang, Ya-Dong,et al. Cloning of a novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum[J]. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY,2014,23(1):104-111. |
| APA | Zhang, Hong.,Guo, Yan-Wu.,Yang, Ya-Dong.,Ma, Yi-Jiao.,Liu, Ben-Ye.,...&Ma, Lan-Qing.(2014).Cloning of a novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum.JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY,23(1),104-111. |
| MLA | Zhang, Hong,et al."Cloning of a novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum".JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 23.1(2014):104-111. |
入库方式： OAI收割
来源：植物研究所
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