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Cloning and Characterization of AabHLH1, a bHLH Transcription Factor that Positively Regulates Artemisinin Biosynthesis in Artemisia annua

文献类型：期刊论文

作者Ji, Yunpeng; Xiao, Jingwei2; Shen, Yalin; Ma, Dongming3; Li, Zhenqiu4; Pu, Gaobin3; Li, Xing; Huang, Lili3; Liu, Benye3; Ye, Hechun3
刊名PLANT AND CELL PHYSIOLOGY
出版日期2014
卷号55期号:9页码:1592-1604
关键词Amorpha-4,11-diene synthase (ADS) Artemisia annua L Artemisinin biosynthesis bHLH transcription factor Cyt P450 monooxygenase (CYP71AV1)
ISSN号0032-0781
DOI10.1093/pcp/pcu090
文献子类Article
英文摘要Amorpha-4,11-diene synthase (ADS) and Cyt P450 monooxygenase (CYP71AV1) in Artemisia annua L. are two key enzymes involved in the biosynthesis of artemisinin. The promoters of ADS and CYP71AV1 contain E-box elements, which are putative binding sites for basic helix-loop-helix (bHLH) transcription factors. This study successfully isolated a bHLH transcription factor gene from A. annua, designated as AabHLH1, from a cDNA library of the glandular secretory trichomes (GSTs) in which artemisinin is synthesized and sequestered. AabHLH1 encodes a protein of 650 amino acids containing one putative bHLH domain. AabHLH1 and ADS genes were strongly induced by ABA and the fungal elicitor, chitosan. The transient expression analysis of the AabHLH1-green fluorescent protein (GFP) reporter gene revealed that AabHLH1 was targeted to nuclei. Biochemical analysis demonstrated that the AabHLH1 protein was capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possessed transactivation activity in yeast. In addition, transient co-transformation of AabHLH1 and CYP71AV1Pro::GUS in A. annua leaves showed a significant activation of the expression of the GUS (beta-glucuronidase) gene in transformed A. annua, but mutation of the E-boxes resulted in abolition of activation, suggesting that the E-box is important for the CYP71AV1 promoter activity. Furthermore, transient expression of AabHLH1 in A. annua leaves increased transcript levels of the genes involved in artemisinin biosynthesis, such as ADS, CYP71AV1 and HMGR. These results suggest that AabHLH1 can positively regulate the biosynthesis of artemisinin.
学科主题Plant Sciences ; Cell Biology
出版地OXFORD
电子版国际标准刊号1471-9053
WOS关键词AMORPHA-4,11-DIENE SYNTHASE ; ALKALOID BIOSYNTHESIS ; MOLECULAR-CLONING ; KEY ENZYME ; EXPRESSION ; GENE ; ACID ; PROTEIN ; IDENTIFICATION ; LOCALIZATION
WOS研究方向Plant Sciences ; Cell Biology
语种英语
WOS记录号WOS:000342978300007
出版者OXFORD UNIV PRESS
资助机构National Natural Science Foundation of China [61173098, 61379081] ; Chinese Academy of Sciences [KSCX2-EW-J-29]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27088]  
专题中科院植物分子生理学重点实验室
作者单位1.Hebei Univ, Baoding 071002, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Natl Inst Occupat Hlth & Poison Control, Beijing 100050, Peoples R China
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Ji, Yunpeng,Xiao, Jingwei,Shen, Yalin,et al. Cloning and Characterization of AabHLH1, a bHLH Transcription Factor that Positively Regulates Artemisinin Biosynthesis in Artemisia annua[J]. PLANT AND CELL PHYSIOLOGY,2014,55(9):1592-1604.
APA Ji, Yunpeng.,Xiao, Jingwei.,Shen, Yalin.,Ma, Dongming.,Li, Zhenqiu.,...&Wang, Hong.(2014).Cloning and Characterization of AabHLH1, a bHLH Transcription Factor that Positively Regulates Artemisinin Biosynthesis in Artemisia annua.PLANT AND CELL PHYSIOLOGY,55(9),1592-1604.
MLA Ji, Yunpeng,et al."Cloning and Characterization of AabHLH1, a bHLH Transcription Factor that Positively Regulates Artemisinin Biosynthesis in Artemisia annua".PLANT AND CELL PHYSIOLOGY 55.9(2014):1592-1604.

入库方式： OAI收割

来源：植物研究所

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