OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4
文献类型：期刊论文
| 作者 | Liu, Huanhuan; Guo, Siyi; Xu, Yunyuan; Li, Chunhua; Zhang, Zeyong; Zhang, Dajian; Xu, Shujuan; Zhang, Cui ; Chong, Kang1
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| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 165期号:1页码:160-174 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.114.235564 |
| 文献子类 | Article |
| 英文摘要 | Inflorescence and spikelet development determine grain yields in cereals. Although multiple genes are known to be involved in the regulation of floral organogenesis, the underlying molecular network remains unclear in cereals. Here, we report that the rice (Oryza sativa) microRNA396d (OsmiR396d) and its Os Growth Regulating Factor (OsGRF) targets, together with Os Growth Regulating Factor-Interacting Factor1 (OsGIF1), are involved in the regulation of floral organ development through the rice JMJD2 family jmjC gene 706 (OsJMJ706) and crinkly4 receptor-like kinase (OsCR4). Transgenic knockdown lines of OsGRF6, a predicted target of OsmiR396d, and overexpression lines of OsmiR396d showed similar defects in floral organ development, including open husks, long sterile lemmas, and altered floral organ morphology. These defects were almost completely rescued by overexpression of OsGRF6. OsGRF6 and its ortholog OsGRF10 were the most highly expressed OsGRF family members in young inflorescences, and the grf6/grf10 double mutant displayed abnormal florets. OsGRF6/OsGRF10 localized to the nucleus, and electrophoretic mobility shift assays revealed that both OsGRF6 and OsGRF10 bind the GA response element in the promoters of OsJMJ706 and OsCR4, which were reported to participate in the regulation of floral organ development. In addition, OsGRF6 and OsGRF10 could transactivate OsJMJ706 and OsCR4, an activity that was enhanced in the presence of OsGIF1, which can bind both OsGRF6 and OsGRF10. Together, our results suggest that OsmiR396d regulates the expression of OsGRF genes, which function with OsGIF1 in floret development through targeting of JMJ706 and OsCR4. This work thus reveals a microRNA-mediated regulation module for controlling spikelet development in rice. |
| 学科主题 | Plant Sciences |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | MADS-BOX GENE ; ARABIDOPSIS-THALIANA ; CELL-PROLIFERATION ; ECTOPIC EXPRESSION ; ORGAN IDENTITY ; MERISTEM FATE ; TRANSCRIPTION FACTORS ; SPIKELET DEVELOPMENT ; FLOWER DEVELOPMENT ; HOMEOTIC GENE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000335906300013 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Natural Sciences Foundation of China [31270331, 31121065] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27089]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res Beijing, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Huanhuan,Guo, Siyi,Xu, Yunyuan,et al. OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4[J]. PLANT PHYSIOLOGY,2014,165(1):160-174. |
| APA | Liu, Huanhuan.,Guo, Siyi.,Xu, Yunyuan.,Li, Chunhua.,Zhang, Zeyong.,...&Chong, Kang.(2014).OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4.PLANT PHYSIOLOGY,165(1),160-174. |
| MLA | Liu, Huanhuan,et al."OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4".PLANT PHYSIOLOGY 165.1(2014):160-174. |
入库方式： OAI收割
来源：植物研究所
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