Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
文献类型：期刊论文
| 作者 | Hao, Huaiqing; Fan, Lusheng; Chen, Tong; Li, Ruili1,2; Li, Xiaojuan1,2; He, Qihua3; Botella, Miguel A.; Lin, Jinxing1,2 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2014 |
| 卷号 | 26期号:4页码:1729-1745 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.113.122358 |
| 文献子类 | Article |
| 英文摘要 | Arabidopsis thaliana respiratory burst oxidase homolog D (RbohD) functions as an essential regulator of reactive oxygen species (ROS). However, our understanding of the regulation of RbohD remains limited. By variable-angle total internal reflection fluorescence microscopy, we demonstrate that green fluorescent protein (GFP)-RbohD organizes into dynamic spots at the plasma membrane. These RbohD spots have heterogeneous diffusion coefficients and oligomerization states, as measured by photobleaching techniques. Stimulation with ionomycin and calyculin A, which activate the ROS-producing enzymatic activity of RbohD, increases the diffusion and oligomerization of RbohD. Abscisic acid and flg22 treatments also increase the diffusion coefficient and clustering of GFP-RbohD. Single-particle analysis in clathrin heavy chain2 mutants and a Flotillin1 artificial microRNA line demonstrated that clathrin-and microdomain-dependent endocytic pathways cooperatively regulate RbohD dynamics. Under salt stress, GFP-RbohD assembles into clusters and then internalizes into the cytoplasm. Dual-color fluorescence cross-correlation spectroscopy analysis further showed that salt stress stimulates RbohD endocytosis via membrane microdomains. We demonstrate that microdomain-associated RbohD spots diffuse at the membrane with high heterogeneity, and these dynamics closely relate to RbohD activity. Our results provide insight into the regulation of RbohD activity by clustering and endocytosis, which facilitate the activation of redox signaling pathways. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-298X |
| WOS关键词 | RESPIRATORY BURST OXIDASE ; REFLECTION FLUORESCENCE MICROSCOPY ; DETERGENT-RESISTANT MEMBRANES ; POSITIVE FEEDBACK-REGULATION ; SINGLE-MOLECULE DIFFUSION ; REVEALS MULTIPLE-MODES ; LIPID RAFTS ; PROTEIN ; ENDOCYTOSIS ; ATRBOHD |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000337351300026 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Basic Research Program of China (973 Program) [2011CB944600, 2011CB809103] ; National Natural Science Foundation of China [31121065, 31170163, 31270224] ; Program of Introducing Talents of Discipline to Universities (111 project) [B13007] ; Program for Changjiang Scholars and Innovative Research Team in University [IRT 13047] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27099]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 4.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 5.Botella, Miguel A.] Univ Malaga, Dept Biol Celular Genet & Fisiol, E-29071 Malaga, Spain |
| 推荐引用方式 GB/T 7714 | Hao, Huaiqing,Fan, Lusheng,Chen, Tong,et al. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis[J]. PLANT CELL,2014,26(4):1729-1745. |
| APA | Hao, Huaiqing.,Fan, Lusheng.,Chen, Tong.,Li, Ruili.,Li, Xiaojuan.,...&Lin, Jinxing.(2014).Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis.PLANT CELL,26(4),1729-1745. |
| MLA | Hao, Huaiqing,et al."Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis".PLANT CELL 26.4(2014):1729-1745. |
入库方式： OAI收割
来源：植物研究所
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