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AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana

文献类型：期刊论文

作者Yu, Qing-Bo; Ma, Qian; Kong, Meng-Meng; Zhao, Tuan-Tuan; Zhang, Xiao-Lei; Zhou, Que; Huang, Chao; Chong, Kang2; Yang, Zhong-Nan
刊名MOLECULAR PLANT
出版日期2014
卷号7期号:1页码:206-217
关键词Arabidopsis Thioredoxin disulfide reductase activity chloroplast transcription chloroplast biogenesis
ISSN号1674-2052
DOI10.1093/mp/sst092
文献子类Article
英文摘要AtECB1/MRL7 with disulfide reductase activity in vitro can interact with Trx Z and FSD3, two intrinsic subunits of plastid-encoded RNA polymerase (PEP) complex. It may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development.Plastid-encoded RNA polymerase (PEP) is closely associated with numerous factors to form PEP complex for plastid gene expression and chloroplast development. However, it is not clear how PEP complex are regulated in chloroplast. Here, one thioredoxin-like fold protein, Arabidopsis early chloroplast biogenesis 1 (AtECB1), an allele of MRL7, was identified to regulate PEP function and chloroplast biogenesis. The knockout lines for AtECB1 displayed albino phenotype and impaired chloroplast development. The transcripts of PEP-dependent plastid genes were barely detected, suggesting that the PEP activity is almost lost in atecb1-1. Although AtECB1 was not identified in PEP complex, a yeast two-hybrid assay and pull-down experiments demonstrated that it can interact with Trx Z and FSD3, two intrinsic subunits of PEP complex, respectively. This indicates that AtECB1 may play a regulatory role for PEP-dependent plastid gene expression through these two subunits. AtECB1 contains a structure in the thioredoxin-like fold domain and lacks the typical C-X-X-C active site motif. Insulin assay demonstrated that AtECB1 harbors disulfide reductase activity in vitro using the purified recombinant AtECB1 protein. This showed that this thioredoxin-like fold protein, AtECB1 also has the thioredoxin activity. AtECB1 may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
出版地CAMBRIDGE
电子版国际标准刊号1752-9867
WOS关键词RNA-POLYMERASE ; CHROMOSOME COMPLEX ; REDOX REGULATION ; REPEAT PROTEIN ; TRANSCRIPTION ; INTERACTS ; GROWTH ; INTEGRATION ; COMPONENT ; PROTEOMES
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000329254200015
出版者CELL PRESS
资助机构Shanghai Municipal Natural Science Foundation [10ZR1421800] ; National Science Foundation of China [31100965] ; National Basic Research Program of China [2009CB118504] ; Young Teachers Training Program of Shanghai Municipal Education Commission [shsf021] ; Leading Academic Discipline Project of the Shanghai Municipal Education Commission [J50401]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27118]  
专题中科院植物分子生理学重点实验室
作者单位1.Shanghai Normal Univ, Dept Biol, Life & Environm Coll, Shanghai 200234, Peoples R China
2.Chinese Acad Sci, Natl Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China
3.Chinese Acad Sci, Res Ctr Mol & Dev Biol, Key Lab Photosynth & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Qing-Bo,Ma, Qian,Kong, Meng-Meng,et al. AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana[J]. MOLECULAR PLANT,2014,7(1):206-217.
APA Yu, Qing-Bo.,Ma, Qian.,Kong, Meng-Meng.,Zhao, Tuan-Tuan.,Zhang, Xiao-Lei.,...&Yang, Zhong-Nan.(2014).AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana.MOLECULAR PLANT,7(1),206-217.
MLA Yu, Qing-Bo,et al."AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana".MOLECULAR PLANT 7.1(2014):206-217.

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来源：植物研究所

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