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The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis

文献类型：期刊论文

作者Qin, Zhixiang; Zhang, Xiaoran1; Zhang, Xiao1; Xin, Wei; Li, Jia2; Hu, Yuxin3
刊名JOURNAL OF EXPERIMENTAL BOTANY
出版日期2014
卷号65期号:9页码:2521-2531
关键词Arabidopsis BRP4 male gametogenesis mitosis ORC6 TFIIB-related protein
ISSN号0022-0957
DOI10.1093/jxb/eru140
文献子类Article
英文摘要Male gametogenesis in angiosperms involves two rounds of mitosis that are essential for the generation of two sperm cells to achieve double fertilization, a distinct event in the sexual reproduction of flowering plants. Precise regulation of mitosis during male gametogenesis is critically important for the establishment of the male germline. However, the molecular mechanisms underlying mitotic division during male gametophyte development have not been characterized fully. Here, we report that the Arabidopsis transcription initiation factor TFIIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis. BRP4 was expressed predominately in developing male gametophytes. Knockdown expression of BRP4 by a native promoter-driven RNA interference construct in Arabidopsis resulted in arrest of the mitotic progression of male gametophytes, leading to a defect in pollen development. Moreover, we showed that the level of expression of a gene encoding a subunit of the origin recognition complex, ORC6, was decreased in BRP4 knockdown plants, and that the ORC6 knockdown transgenic plants phenocopied the male gametophyte defect observed in BRP4 knockdown plants, suggesting that ORC6 acts downstream of BRP4 to mediate male mitotic progression. Taken together, our results reveal that BRP4 plays an important role in the regulation of mitotic cell-cycle progression during male gametogenesis.
学科主题Plant Sciences
出版地OXFORD
电子版国际标准刊号1460-2431
WOS关键词MALE GAMETOPHYTE DEVELOPMENT ; TFIIB-RELATED PROTEIN ; ORIGIN RECOGNITION COMPLEX ; GENE-EXPRESSION ; ORGAN GROWTH ; E3 LIGASES ; POLLEN ; THALIANA ; PLANTS ; DIVISION
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000337978800024
出版者OXFORD UNIV PRESS
资助机构National Natural Science Foundation of China [30800599, 31121065] ; Ministry of Science and Technology of China [2009CB941500]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27144]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
4.Natl Ctr Plant Gene Res, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Qin, Zhixiang,Zhang, Xiaoran,Zhang, Xiao,et al. The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(9):2521-2531.
APA Qin, Zhixiang,Zhang, Xiaoran,Zhang, Xiao,Xin, Wei,Li, Jia,&Hu, Yuxin.(2014).The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis.JOURNAL OF EXPERIMENTAL BOTANY,65(9),2521-2531.
MLA Qin, Zhixiang,et al."The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis".JOURNAL OF EXPERIMENTAL BOTANY 65.9(2014):2521-2531.

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来源：植物研究所

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