Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis
文献类型：期刊论文
| 作者 | Wang, Zizhang; Xue, Zhen; Wang, Tai
|
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 13期号:9页码:3987-4001 |
| 关键词 | Proteomics metabolomics principal component analysis additively balanced networking heterosis maize |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr500337j |
| 文献子类 | Article |
| 英文摘要 | A century ago, dominance and overdominance hypotheses were developed to explain the phenomenon of heterosis, both hypotheses were in a nonadditive pattern. Here, a principal component analysis (PCA) of maize seed proteomes was used for representative inbreds of five heterotic germplasms and three classes of hybrid. Hybrids congregated in the center region of inbreds, forming an additive distribution with hybrids in the middle of their parents. Principal components 1 and 2 indicated biased distributions of proteins with functions of amino acid protein or carbohydrate energy metabolisms, respectively, after loading analysis and MS identification of proteins. Then, GC MS was used to examine free amino acids, carbohydrates, and organic acids. A lower level of these metabolites were found in hybrids than inbreds. Further, we performed similar analyses of germinating seeds of a parent-F1 triad and three F2 segregants and confirmed these results. Therefore, an additive pattern of protein abundances for an unimpeded flow of metabolites was established in heterotic hybrids. That is, an additively balanced networking but not the nonadditive dominance or overdominance regulates heterosis. The less expensive metabolism in hybrids suggested the evolution of sexual reproduction. The Mendelian phenotypic ratio can be better explained based on this additive pattern than dominance. |
| 学科主题 | Biochemical Research Methods |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1535-3907 |
| WOS关键词 | NONADDITIVE PROTEIN ACCUMULATION ; MAYS L. F-1-HYBRID ; GENE-EXPRESSION ; INBRED LINES ; GROWTH ; PATTERNS ; RICE ; HETEROZYGOSITY ; DIVERSITY ; HYBRIDS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000341345000012 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | China Ministry of Agriculture [2008-2013ZX08012-002] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27198]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zizhang,Xue, Zhen,Wang, Tai. Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(9):3987-4001. |
| APA | Wang, Zizhang,Xue, Zhen,&Wang, Tai.(2014).Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis.JOURNAL OF PROTEOME RESEARCH,13(9),3987-4001. |
| MLA | Wang, Zizhang,et al."Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis".JOURNAL OF PROTEOME RESEARCH 13.9(2014):3987-4001. |
入库方式： OAI收割
来源：植物研究所
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