Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system
文献类型：期刊论文
| 作者 | Chen, Xianyang; Bao, Hexigeduleng; Guo, Jie; Jia, Weitao; Tai, Fang; Nie, Lingling; Jiang, Ping; Feng, Juanjuan; Lv, Sulian; Li, Yinxin
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| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2014 |
| 卷号 | 65期号:20页码:6107-6122 |
| 关键词 | Cellular redox homeostasis disease resistance NAD(P) (H) pool NHX1 tobacco vacuolar H+ flux and pH |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/eru351 |
| 文献子类 | Article |
| 英文摘要 | Despite the importance of NHX1 (Na+/H+ exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of NbNHX1 with its C-terminus fused with green fluorescent protein indicated that NbNHX1 localized primarily to the tonoplast. Tobacco rattle virus-induced NbNHX1 silencing led to reduced H+ efflux from the vacuole to cytoplasts, and decreased Ppn resistance in N. benthamiana. After attack by Ppn, NbNHX1-silenced plants exhibited impaired ability to scavenge reactive oxidative species (ROS) induced by the pathogen. Pea early browning virus-mediated ectopic expression of SeNHX1 (from Salicornia europaea) or AtNHX1 (from Arabidopsis thaliana) both conferred enhanced Ppn resistance to N. benthamiana, with a lower H2O2 concentration after Ppn inoculation. Further investigation of the role of NHX1 demonstrated that transient overexpression of NbNHX1 improved the vacuolar pH and cellular ROS level in N. benthamiana, which was coupled with an enlarged NAD(P) (H) pool and higher expression of ROS-responsive genes. In contrast, NbNHX1 silencing led to a lower pH in the vacuole and a lower cellular ROS level in N. benthamiana, which was coupled with a decreased NAD(P) (H) pool and decreased expression of ROS-responsive genes. These results suggest that NHX1 is involved in plant disease defence; and regulation of vacuolar pH by NHX1, affecting the cellular oxidation state, primes the antioxidative system which is associated with Ppn resistance in tobacco. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | CONFERS SALT-TOLERANCE ; REACTIVE OXYGEN ; ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; GENE-EXPRESSION ; ANTIPORTER GENE ; CELL-DEATH ; HOMEOSTASIS ; OVEREXPRESSION ; PLANTS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000344658800032 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Chinese Ministry of Agriculture [2013ZX08009-003-002] ; National Natural Science Foundation of China [31200201] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27256]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xianyang,Bao, Hexigeduleng,Guo, Jie,et al. Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(20):6107-6122. |
| APA | Chen, Xianyang.,Bao, Hexigeduleng.,Guo, Jie.,Jia, Weitao.,Tai, Fang.,...&Li, Yinxin.(2014).Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system.JOURNAL OF EXPERIMENTAL BOTANY,65(20),6107-6122. |
| MLA | Chen, Xianyang,et al."Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system".JOURNAL OF EXPERIMENTAL BOTANY 65.20(2014):6107-6122. |
入库方式： OAI收割
来源：植物研究所
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