Effects of silver nitrate on shoot regeneration of Artemisia annua L.
文献类型:期刊论文
作者 | Lei, Caiyan1,2; Wang, Hong2; Liu, Benye1; Ye, Hechun1 |
刊名 | PLANT BIOTECHNOLOGY
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出版日期 | 2014 |
卷号 | 31期号:1页码:71-75 |
关键词 | Artemisia annua L. elongation growth shoot induction silver nitrate |
ISSN号 | 1342-4580 |
DOI | 10.5511/plantbiotechnology.13.1109a |
文献子类 | Article |
英文摘要 | In order to further increase the shoot regeneration frequency of Artemisia annua L., the effects of silver nitrate on this process was investigated in this study. Different concentration of silver nitrate was added to the shoot induction medium, which was MS basic medium containing 1.0 mgl(-1) 6-benzyladenine (6-BA) and 0.05 mgl(-1) alpha-napthaleneacetic acid (NAA). When 2 mgl(-1) silver nitrate was added to the shoot induction medium, the shoot induction frequency and shoot number per explants was significantly higher than that of the control (without silver nitrate). In addition, silver nitrate at all the tested concentrations could significantly reduce callus formation of the explants. Silver nitrate had also positive influence on shoot elongation in the first 20 days. Furthermore, silver nitrate did not affect the sensitivity of A. annua shoots to Kanamycin (KM); therefore, silver nitrate could be used to improve shoot regeneration capacity and frequency in A. annua genetic transformation. |
学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
出版地 | NARA |
WOS关键词 | TUMEFACIENS-MEDIATED TRANSFORMATION ; PLANT-REGENERATION ; BIOSYNTHESIS ; IMPROVEMENT ; CULTIVARS ; ETHYLENE ; GROWTH |
WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
语种 | 英语 |
WOS记录号 | WOS:000332467700011 |
出版者 | JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY |
资助机构 | Public Welfare (agriculture) Research Project China [201303030] ; Science and Technology Research Projects of Henan Province, China [13B210049] |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27273] ![]() |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Lei, Caiyan,Wang, Hong,Liu, Benye,et al. Effects of silver nitrate on shoot regeneration of Artemisia annua L.[J]. PLANT BIOTECHNOLOGY,2014,31(1):71-75. |
APA | Lei, Caiyan,Wang, Hong,Liu, Benye,&Ye, Hechun.(2014).Effects of silver nitrate on shoot regeneration of Artemisia annua L..PLANT BIOTECHNOLOGY,31(1),71-75. |
MLA | Lei, Caiyan,et al."Effects of silver nitrate on shoot regeneration of Artemisia annua L.".PLANT BIOTECHNOLOGY 31.1(2014):71-75. |
入库方式: OAI收割
来源:植物研究所
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