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Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness

文献类型：期刊论文

作者Meng, Hongyan; Jiang, Ling1; Xu, Bosi; Guo, Wenzhu2; Li, Jinglai3; Zhu, Xiuqing3; Qi, Xiaoquan4; Duan, Lixin4; Meng, Xianbin4; Fan, Yunliu1
刊名PLOS ONE
出版日期2014
卷号9期号:7
ISSN号1932-6203
DOI10.1371/journal.pone.0101905
文献子类Article
英文摘要Interactions among metabolic pathways are important in plant biology. At present, not much is known about how folate metabolism affects other metabolic pathways in plants. Here we report a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) was defective in seed reserves and skotomorphogenesis. Lower carbon (C) and higher nitrogen (N) content in the mutant seeds than that of the wild type were indicative of an altered C and N partitioning capacity. Higher levels of organic acids and sugars were detected in the mutant seeds compared with the wild type. Further analysis revealed that atdfb-3 seeds contained less total amino acids and individual Asn and Glu as well as NO3-. These results indicate significant changes in seed storage in the mutant. Defects in hypocotyl elongation were observed in atdfb-3 in darkness under sufficient NO3- conditions, and further enhanced under NO3- limited conditions. The strong expression of AtDFB in cotyledons and hypocotyl during early developmental stage was consistent with the mutant sensitivity to limited NO3- during a narrow developmental window. Exogenous 5-formyl-tetrahydrofolate completely restored the hypocotyl length in atdfb-3 seedlings with NO3- as the sole N source. Further study demonstrated that folate profiling and N metabolism were perturbed in atdfb-3 etiolated seedlings. The activity of enzymes involved in N reduction and assimilation was altered in atdfb-3. Taken together, these results indicate that AtDFB is required for seed reserves, hypocotyl elongation and N metabolism in darkness, providing novel insights into potential associations of folate metabolism with seed reserve accumulation, N metabolism and hypocotyl development in Arabidopsis.
学科主题Multidisciplinary Sciences
出版地SAN FRANCISCO
WOS关键词ONE-CARBON METABOLISM ; PISUM-SATIVUM-L ; HIGHER-PLANTS ; TETRAHYDROFOLATE BIOSYNTHESIS ; FOLATE POLYGLUTAMYLATION ; CELL ELONGATION ; GERMINATION ; THALIANA ; GROWTH ; ACID
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000338637300100
出版者PUBLIC LIBRARY SCIENCE
资助机构National Basic Research Program of China [2013CB127003]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27315]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China
2.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China
3.Huazhong Agr Univ, Wuhan, Peoples R China
4.Beijing Inst Pharmacol & Toxicol, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Meng, Hongyan,Jiang, Ling,Xu, Bosi,et al. Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness[J]. PLOS ONE,2014,9(7).
APA Meng, Hongyan.,Jiang, Ling.,Xu, Bosi.,Guo, Wenzhu.,Li, Jinglai.,...&Zhang, Chunyi.(2014).Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness.PLOS ONE,9(7).
MLA Meng, Hongyan,et al."Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness".PLOS ONE 9.7(2014).

入库方式： OAI收割

来源：植物研究所

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