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Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing

文献类型：期刊论文

作者Zhang, Fan1; Tang, Weijiang; Hedtke, Boris2; Zhong, Linlin1; Liu, Lin; Peng, Lianwei; Lu, Congming3; Grimm, Bernhard2; Lin, Rongcheng3
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2014
卷号111期号:5页码:2023-2028
关键词metabolism organelle editosome
ISSN号0027-8424
DOI10.1073/pnas.1316183111
文献子类Article
英文摘要RNA editing is a posttranscriptional process that covalently alters the sequence of RNA molecules and plays important biological roles in both animals and land plants. In flowering plants, RNA editing converts specific cytidine residues to uridine in both plastid and mitochondrial transcripts. Previous studies identified pentatricopeptide repeat (PPR) motif-containing proteins as site-specific recognition factors for cytidine targets in RNA sequences. However, the regulatory mechanism underlying RNA editing was largely unknown. Here, we report that protoporphyrinogen IX oxidase 1 (PPO1), an enzyme that catalyzes protoporphyrinogen IX into protoporphyrin IX in the tetrapyrrole biosynthetic pathway, plays an unexpected role in editing multiple sites of plastid RNA transcripts, most of which encode subunits of the NADH dehydrogenase-like complex (NDH), in the reference plant Arabidopsis thaliana. We identified multiple organellar RNA editing factors (MORFs), including MORF2, MORF8, and MORF9, that interact with PPO1. We found that two conserved motifs within the 22-aa region at the N terminus of PPO1 are essential for its interaction with MORFs, its RNA editing function, and subsequently, its effect on NDH activity. However, transgenic plants lacking key domains for the tetrapyrrole biosynthetic activity of PPO1 exhibit normal RNA editing. Furthermore, MORF2 and MORF9 interact with three PPRs or related proteins required for editing of ndhB and ndhD sites. These results reveal that the tetrapyrrole biosynthetic enzyme PPO1 is required for plastid RNA editing, acting as a regulator that promotes the stability of MORF proteins through physical interaction.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEINS ; PPR PROTEINS ; ARABIDOPSIS ; CHLOROPLAST ; REVEALS ; SITE ; DISRUPTION ; INTERACTS ; MOTIF
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000330587600082
出版者NATL ACAD SCIENCES
资助机构National Natural Science Foundation of China [31170221] ; Chinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27114]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Humboldt Univ, Inst Biol, Dept Plant Physiol, D-10099 Berlin, Germany
4.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Fan,Tang, Weijiang,Hedtke, Boris,et al. Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014,111(5):2023-2028.
APA Zhang, Fan.,Tang, Weijiang.,Hedtke, Boris.,Zhong, Linlin.,Liu, Lin.,...&Lin, Rongcheng.(2014).Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,111(5),2023-2028.
MLA Zhang, Fan,et al."Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.5(2014):2023-2028.

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来源：植物研究所

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