Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis
文献类型：期刊论文
| 作者 | Liu, Cheng; Rao, Yan; Zhang, Lei; Yang, Chunhong |
| 刊名 | JOURNAL OF BIOCHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 156期号:4页码:203-210 |
| 关键词 | photosynthesis aggregation hydrophobicity light-harvesting chlorophyll a/b complexes of photosystem II photosynthesis stability |
| ISSN号 | 0021-924X |
| DOI | 10.1093/jb/mvu028 |
| 文献子类 | Article |
| 英文摘要 | The functions of the helix E (W97-F105), an amphiphilic lumenal 310 helix of the major antenna of photosystem II (LHCII), are still unidentified. To elucidate the roles of individual amino acid residue of the helix E, alanine scanning mutagenesis has been performed to mutate every residue of this domain to alanine. The influence of every alanine substitution on the structure and function of LHCII has been investigated biochemically and spectroscopically. The results show that all mutations have little impact on the pigment binding and configuration. However, many mutants presented decreased thermo-or photo-stability compared with the wild type, highlighting the significance of this helix to the stability of LHCII. The most critical residue for stability is W97. The mutant W97A yielded very fragile trimeric pigment protein complexes. The structural analysis revealed that the hydrogen bonding and aromatic interactions between W97, F195, F194 and a water molecule contributed greatly to the stability of LHCII. Moreover, Q103A and F105A have been identified to be able to reinforce the tendency of aggregation in vitro. The structural analysis suggested that the enhancement in aggregation formation for Q103A and F105A might be attributed to the changing hydrophobicity of the region. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1756-2651 |
| WOS关键词 | LIGHT-HARVESTING COMPLEX ; CHLOROPHYLL-A/B-COMPLEX ; PIGMENT-BINDING ; LUMENAL LOOP ; IN-VITRO ; XANTHOPHYLL CYCLE ; CRYSTAL-STRUCTURE ; HIGHER-PLANTS ; PROTEIN ; MECHANISM |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000343324700003 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Basic Research Program of China [2011CBA00904] ; Key Research Program of the Chinese Academy of Sciences [KSZD-EW-Z-018] ; National Natural Science Foundation of China [30800069, 31070212, 31370275] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27115]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Cheng,Rao, Yan,Zhang, Lei,et al. Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis[J]. JOURNAL OF BIOCHEMISTRY,2014,156(4):203-210. |
| APA | Liu, Cheng,Rao, Yan,Zhang, Lei,&Yang, Chunhong.(2014).Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis.JOURNAL OF BIOCHEMISTRY,156(4),203-210. |
| MLA | Liu, Cheng,et al."Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis".JOURNAL OF BIOCHEMISTRY 156.4(2014):203-210. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。