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Functional Proteomic Discovery of Slr0110 as a Central Regulator of Carbohydrate Metabolism in Synechocystis Species PCC6803

文献类型：期刊论文

作者Gao, Liyan; Shen, Chunting; Liao, Libing1; Huang, Xiahe; Liu, Kehui; Wang, Wei; Guo, Lihai2; Jin, Wenhai2; Huang, Fang1; Xu, Wu3
刊名MOLECULAR & CELLULAR PROTEOMICS
出版日期2014
卷号13期号:1页码:204-219
ISSN号1535-9476
DOI10.1074/mcp.M113.033803
文献子类Article
英文摘要The unicellular photosynthetic model-organism cyanobacterium Synechocystis sp. PCC6803 can grow photoautotrophically using CO2 or heterotrophically using glucose as the sole carbon source. Several pathways are involved in carbon metabolism in Synechocystis, and the concerted regulation of these pathways by numerous known and unknown genes is critical for the survival and growth of the organism. Here, we report that a hypothetical protein encoded by the open reading frame slr0110 is necessary for heterotrophic growth of Synechocystis. The slr0110-deletion mutant is defective in glucose uptake, heterotrophic growth, and dark viability without detectable defects in autotrophic growth, whereas the level of photosystem II and the rate of oxygen evolution are increased in the mutant. Quantitative proteomic analysis revealed that several proteins in glycolysis and the oxidative pentose phosphate pathway are down-regulated, whereas proteins in photosystem II and phycobilisome are significantly up-regulated, in the mutant. Among the down-regulated proteins are glucose transporter, glucokinase, glucose-6-phosphate isomerase, and glucose-6-phosphate dehydrogenase and its assembly protein OpcA, suggesting that glycolysis, oxidative pentose phosphate, and glycogen synthesis pathways are significantly inhibited in the mutant, which was further confirmed by enzymatic assays and quantification of glycogen content. These findings establish Slr0110 as a novel central regulator of carbon metabolism in Synechocystis, and shed light on an intricate mechanism whereby photosynthesis and carbon metabolism are well concerted to survive the crisis when one or more pathways of the system are impaired.
学科主题Biochemical Research Methods
出版地ROCKVILLE
电子版国际标准刊号1535-9484
WOS关键词SP STRAIN PCC-6803 ; PCC 6803 ; ELECTRON-TRANSPORT ; GENE-EXPRESSION ; GENOME DATABASE ; PHOTOSYSTEM-I ; PROTEIN SPOTS ; CYANOBACTERIUM ; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ; ENRICHMENT
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000329343000016
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
资助机构Ministry of Science and Technology of China [2012CB910900, 2011CB915402] ; National Science Foundation of China [31300677]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27117]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
3.AB Sciex China, Beijing 100015, Peoples R China
4.Univ Louisiana Lafayette, Dept Chem, Lafayette, LA 70504 USA
推荐引用方式
GB/T 7714		 Gao, Liyan,Shen, Chunting,Liao, Libing,et al. Functional Proteomic Discovery of Slr0110 as a Central Regulator of Carbohydrate Metabolism in Synechocystis Species PCC6803[J]. MOLECULAR & CELLULAR PROTEOMICS,2014,13(1):204-219.
APA Gao, Liyan.,Shen, Chunting.,Liao, Libing.,Huang, Xiahe.,Liu, Kehui.,...&Wang, Yingchun.(2014).Functional Proteomic Discovery of Slr0110 as a Central Regulator of Carbohydrate Metabolism in Synechocystis Species PCC6803.MOLECULAR & CELLULAR PROTEOMICS,13(1),204-219.
MLA Gao, Liyan,et al."Functional Proteomic Discovery of Slr0110 as a Central Regulator of Carbohydrate Metabolism in Synechocystis Species PCC6803".MOLECULAR & CELLULAR PROTEOMICS 13.1(2014):204-219.

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来源：植物研究所

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