The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution
文献类型：期刊论文
| 作者 | Zhang, Lei1; Melo, Thor Bernt; Li, Heng; Naqvi, K. Razi; Yang, Chunhong |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 171期号:5页码:42-48 |
| 关键词 | Light-harvesting chlorophyll a/b complexes Conformation Chlorophyll triplet Lutein Mutagenesis |
| ISSN号 | 0176-1617 |
| DOI | 10.1016/j.jplph.2013.11.008 |
| 文献子类 | Article |
| 英文摘要 | Under strong light conditions, long-lived chlorophyll triplets ((3)Chls) are formed, which can sensitize singlet oxygen, a species harmful to the photosynthetic apparatus of plants. Plants have developed multiple photoprotective mechanisms to quench (3)Chl and scavenge singlet oxygen in order to sustain the photosynthetic activities. The lumenal loop of light-harvesting chlorophyll a/b complex of photosystem II (LHCII) plays important roles in regulating the pigment conformation and energy dissipation. In this study, site-directed mutagenesis analysis was applied to investigate triplet-triplet energy transfer and quenching of (3)Chl in LHCII. We mutated the amino acid at site 123 located in this region to Gly, Pro, Gln, Thr and Tyr, respectively, and recorded fluorescence excitation spectra, triplet-minus-singlet (TmS) spectra and kinetics of carotenoid triplet decay for wild type and all the mutants. A red-shift was evident in the TmS spectra of the mutants S123T and S123P, and all of the mutants except S123Y showed a decrease in the triplet energy transfer efficiency. We propose, on the basis of the available structural information, that these phenomena are related to the involvement, due to conformational changes in the lumenal region, of a long-wavelength lutein (Lut2) involved in quenching (3)Chl. (C) 2013 Elsevier GmbH. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | JENA |
| 电子版国际标准刊号 | 1618-1328 |
| WOS关键词 | HIGHER-PLANTS ; BINDING ; XANTHOPHYLLS ; MEMBRANES ; DYNAMICS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000331925900007 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 资助机构 | State Key Basic Research and Development Plan of China [2011CBA00904] ; National Natural Science Foundation of China [31070212, 31370275] ; Research Council of Norway [191102] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27212]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Norwegian Univ Sci & Technol, Dept Phys, N-7491 Trondheim, Norway |
| 推荐引用方式 GB/T 7714 | Zhang, Lei,Melo, Thor Bernt,Li, Heng,et al. The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution[J]. JOURNAL OF PLANT PHYSIOLOGY,2014,171(5):42-48. |
| APA | Zhang, Lei,Melo, Thor Bernt,Li, Heng,Naqvi, K. Razi,&Yang, Chunhong.(2014).The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution.JOURNAL OF PLANT PHYSIOLOGY,171(5),42-48. |
| MLA | Zhang, Lei,et al."The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution".JOURNAL OF PLANT PHYSIOLOGY 171.5(2014):42-48. |
入库方式： OAI收割
来源：植物研究所
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