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RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana

文献类型：期刊论文

作者Chi, Wei; He, Baoye1; Manavski, Nikolay2; Mao, Juan; Ji, Daili; Lu, Congming; Rochaix, Jean David3,4; Meurer, Joerg2; Zhang, Lixin
刊名PLANT CELL
出版日期2014
卷号26期号:12页码:4918-4932
ISSN号1040-4651
DOI10.1105/tpc.114.132118
文献子类Article
英文摘要Although transcription termination is essential to generate functional RNAs, its underlying molecular mechanisms are still poorly understood in plastids of vascular plants. Here, we show that the RNA binding protein RHON1 participates in transcriptional termination of rbcL (encoding large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase) in Arabidopsis thaliana. Inactivation of RHON1 leads to enhanced rbcL read-through transcription and to aberrant accD (encoding beta-subunit of the acetyl-CoA carboxylase) transcriptional initiation, which may result from inefficient transcription termination of rbcL. RHON1 can bind to the mRNA as well as to single-stranded DNA of rbcL, displays an RNA-dependent ATPase activity, and terminates transcription of rbcL in vitro. These results suggest that RHON1 terminates rbcL transcription using an ATP-driven mechanism similar to that of Rho of Escherichia coli. This RHON1-dependent transcription termination occurs in Arabidopsis but not in rice (Oryza sativa) and appears to reflect a fundamental difference between plastomes of dicotyledonous and monocotyledonous plants. Our results point to the importance and significance of plastid transcription termination and provide insights into its machinery in an evolutionary context.
学科主题Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
出版地ROCKVILLE
电子版国际标准刊号1532-298X
WOS关键词PENTATRICOPEPTIDE-REPEAT PROTEIN ; CHLOROPLAST GENE-EXPRESSION ; ENCODED RNA-POLYMERASE ; ESCHERICHIA-COLI ; MESSENGER-RNA ; SIGMA-FACTOR ; NUCLEOTIDE-SEQUENCE ; TOBACCO PLASTIDS ; BINDING ; PLANTS
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种英语
WOS记录号WOS:000348646000028
出版者AMER SOC PLANT BIOLOGISTS
资助机构National Natural Science Foundation of China [31370273] ; Major State Basic Research Development Program [2015CB150100] ; Solar Energy Initiative and Cooperative Team Project of the Chinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27233]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Munich, Biozentrum, D-82152 Planegg Martinsried, Germany
4.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland
5.Univ Geneva, Dept Plant Biol, CH-1211 Geneva, Switzerland
推荐引用方式
GB/T 7714		 Chi, Wei,He, Baoye,Manavski, Nikolay,et al. RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana[J]. PLANT CELL,2014,26(12):4918-4932.
APA Chi, Wei.,He, Baoye.,Manavski, Nikolay.,Mao, Juan.,Ji, Daili.,...&Zhang, Lixin.(2014).RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana.PLANT CELL,26(12),4918-4932.
MLA Chi, Wei,et al."RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana".PLANT CELL 26.12(2014):4918-4932.

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来源：植物研究所

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