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The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region

文献类型：期刊论文

作者Yang, Silu1; Li, Weihua2; Qi, Shaohua1; Gai, Kexin1; Chen, Yibo3; Suo, Jingxia1; Cao, Yingqiong1; He, Yubo4; Wang, Ying1; He, Qun1
刊名NUCLEIC ACIDS RESEARCH
出版日期2014
卷号42期号:10页码:6183-6195
ISSN号0305-1048
DOI10.1093/nar/gku261
文献子类Article
英文摘要In Neurospora crassa, the methionine synthase gene met-8 plays a key role in methionine synthesis. In this study, we found that MET-8 protein levels were compromised in several mutants defective in proper heterochromatin formation. Bioinformatics analysis revealed a 50-kb AT-rich region adjacent to the met-8 promoter. ChIP assays confirmed that trimethylated H3K9 was enriched in this region, indicating that heterochromatin may form upstream of met-8. In an H3K9R mutant strain, the output of met-8 was dramatically reduced, similar to what we observed in mutant strains that had defective heterochromatin formation. Furthermore, the production of ectopically expressed met-8 at the his-3 locus in the absence of a normal heterochromatin environment was inefficient, whereas ectopic expression of met-8 downstream of two other heterochromatin domains was efficient. In addition, our data show that the expression of mig-6 was also controlled by an upstream 4.2-kb AT-rich region similar to that of the met-8 gene, and we demonstrate that the AT-rich regions adjacent to met-8 or mig-6 are required for their peak expression. Our study indicates that met-8 and mig-6 may represent a novel type of gene, whose expression relies on the proper formation of a nearby heterochromatin region.
学科主题Biochemistry & Molecular Biology
出版地OXFORD
电子版国际标准刊号1362-4962
WOS关键词DROSOPHILA-MELANOGASTER ; UBIQUITIN LIGASE ; DNA METHYLATION ; WD40-REPEAT PROTEINS ; H3 METHYLTRANSFERASE ; EFFECT VARIEGATION ; HP1 ; IDENTIFICATION ; CHROMATIN ; COMPLEX
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000338768100014
出版者OXFORD UNIV PRESS
资助机构National Natural Science Foundation of China [31171208]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27259]  
专题中科院光生物学重点实验室
作者单位1.China Agr Univ, State Key Lab Agrobiotechnol, Coll Biol Sci, Beijing 100193, Peoples R China
2.China Agr Univ, MOA Key Lab Soil Microbiol, Coll Biol Sci, Beijing 100193, Peoples R China
3.Natl Ctr Biomed Anal, Inst Basic Med Sci, Beijing 100850, Peoples R China
4.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
5.Beijing Zhong Guan Cun High Sch, Beijing 100086, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Silu,Li, Weihua,Qi, Shaohua,et al. The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region[J]. NUCLEIC ACIDS RESEARCH,2014,42(10):6183-6195.
APA Yang, Silu.,Li, Weihua.,Qi, Shaohua.,Gai, Kexin.,Chen, Yibo.,...&He, Qun.(2014).The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.NUCLEIC ACIDS RESEARCH,42(10),6183-6195.
MLA Yang, Silu,et al."The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region".NUCLEIC ACIDS RESEARCH 42.10(2014):6183-6195.

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来源：植物研究所

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