Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment
文献类型：期刊论文
| 作者 | Tian, Qi-Lin; Shi, Ding-Ji; Jia, Xiao-Hui; Mi, Hua-Ling; Huang, Xi-Wen; He, Pei-Min |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 36期号:4页码:821-827 |
| 关键词 | Algal lipids Chlamydomonas reinhardtii Escherichia coli Gene expression Lipid production Phosphoenolpyruvate carboxylase Reverse genetics |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-013-1418-9 |
| 文献子类 | Article |
| 英文摘要 | To investigate the function of a bacterial-type phosphoenolpyruvate carboxylase (PEPC2) derived from photosynthetically-grown Chlamydomonas reinhardtii, a fragment of the pepc2 gene was cloned and expressed in Escherichia coli. After optimal induction for 6 h, PEPC activity in the reverse mutant was lower than wild type (0.9 vs. 1.7 U/mg protein), and soluble protein was also lower than wild type (119 vs. 186 mg/g dry wt). In contrast, the total lipid content was increased from 56 (in wild type) to 71 mg/g dry wt, despite the growth rate being slightly diminished. The changes in PEPC activity, soluble protein and total lipid in the forward mutant were the opposite (2.4 U/mg, 230 mg/g, and 44 mg/g dry wt, respectively). Together, these data indicate that PEPC may function as a metabolic pivot in the regulation of protein and lipid accumulation in this alga. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-6776 |
| WOS关键词 | IDENTIFICATION ; EVOLUTION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000333110500019 |
| 出版者 | SPRINGER |
| 资助机构 | National High Technology Research and Development Program of China (863 Program) [2009AA064401] ; Shanghai Universities First-Class Disciplines Project [0707] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27292]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.[Tian, Qi-Lin 2.Shi, Ding-Ji 3.Jia, Xiao-Hui 4.Huang, Xi-Wen 5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China 6.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 7.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Qi-Lin,Shi, Ding-Ji,Jia, Xiao-Hui,et al. Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment[J]. BIOTECHNOLOGY LETTERS,2014,36(4):821-827. |
| APA | Tian, Qi-Lin,Shi, Ding-Ji,Jia, Xiao-Hui,Mi, Hua-Ling,Huang, Xi-Wen,&He, Pei-Min.(2014).Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment.BIOTECHNOLOGY LETTERS,36(4),821-827. |
| MLA | Tian, Qi-Lin,et al."Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment".BIOTECHNOLOGY LETTERS 36.4(2014):821-827. |
入库方式： OAI收割
来源：植物研究所
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