Increasing alpha-linolenic acid content in rice bran by embryo-specific expression of omega 3/Delta 15-desaturase gene
文献类型：期刊论文
| 作者 | Yin, Zhi Jie; Liu, Hua Liang; Dong, Xiangbai; Tian, Lihong; Xiao, Li; Xu, Yi Nong1; Qu, Le Qing |
| 刊名 | MOLECULAR BREEDING
 |
| 出版日期 | 2014 |
| 卷号 | 33期号:4页码:987-996 |
| 关键词 | alpha-Linolenic acid omega 3 Fatty acid desaturase Triacylglycerols Embryo-specific expression Rice bran oil |
| ISSN号 | 1380-3743 |
| DOI | 10.1007/s11032-013-0014-y |
| 文献子类 | Article |
| 英文摘要 | Rice bran, the byproduct of brown rice, with a million tons produced annually, is the major storage organ for lipids. Increasing the alpha-linolenic acid (ALA) content in rice bran by biotechnology strategies is beneficial for both human health and the oil industries. We introduced two omega 3/Delta 15 fatty acid desaturase genes cloned from rice and soybean into rice under the control of an embryo-specific promoter, REG. The ALA content (dry weight) in embryos and bran was increased up to 6.09 and 5.86 mg/g, respectively, in transgenic lines, which was 25.4- and 27.9-fold higher than the 0.24 and 0.21 mg/g in the nontransformant control. ALA accounted for 46.7 and 44.3 % of the total fatty acids in embryos and bran, respectively, of transgenic plants, which was comparable to that in linseed and perilla seeds. The trait of high ALA content was stably inherited. The enhanced ALA content was preferentially located at the sn-2 position in triacylglycerols, which are digestible and absorbable for humans. ALA-enriched rice bran may help alleviate human health problems caused by ALA deficiency, with the production of healthy bran oil for humans and feed for animals. |
| 学科主题 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1572-9788 |
| WOS关键词 | TRANSGENIC RICE ; FATTY-ACID ; SOYBEAN OIL ; ARABIDOPSIS ; SEED ; MUTANTS ; IDENTIFICATION ; ACCUMULATION ; DESATURASES ; PROMOTERS |
| WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000333170800020 |
| 出版者 | SPRINGER |
| 资助机构 | National High Technology Research and Development Program (863) [2011AA100604] ; Natural Science Foundation of China [31301295, 31070226] ; National Program of Transgenic Variety Development of China [2014ZX08001-006] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27309]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Zhi Jie,Liu, Hua Liang,Dong, Xiangbai,et al. Increasing alpha-linolenic acid content in rice bran by embryo-specific expression of omega 3/Delta 15-desaturase gene[J]. MOLECULAR BREEDING,2014,33(4):987-996. |
| APA | Yin, Zhi Jie.,Liu, Hua Liang.,Dong, Xiangbai.,Tian, Lihong.,Xiao, Li.,...&Qu, Le Qing.(2014).Increasing alpha-linolenic acid content in rice bran by embryo-specific expression of omega 3/Delta 15-desaturase gene.MOLECULAR BREEDING,33(4),987-996. |
| MLA | Yin, Zhi Jie,et al."Increasing alpha-linolenic acid content in rice bran by embryo-specific expression of omega 3/Delta 15-desaturase gene".MOLECULAR BREEDING 33.4(2014):987-996. |
入库方式： OAI收割
来源：植物研究所
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